Automated analysis method based on BSA gene positioning

A technology for automated analysis and gene mapping, applied in genomics, instrumentation, proteomics, etc., can solve the problems of unreliable sequencing results, single sequencing samples, and complicated processes, and achieve rich graphical display parts, simplify method processes, and improve The effect of using efficiency

Inactive Publication Date: 2019-02-22
SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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  • Abstract
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Problems solved by technology

[0004] However, the existing BSA analysis methods have defects such as unreliable sequencing results, complicated procedures, and single sequencing samples.
The mutation detection program in the existing process does not use mainstream gene sequencing software, and the results of mutation detection are somewhat different from mainstream software, and the existing process needs to go through several rounds of mutation detection, which is time-consuming
In addition, due to the limitation of sequencing materials, the original process can only handle projects with a single parent

Method used

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  • Automated analysis method based on BSA gene positioning

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Embodiment Construction

[0022] see figure 1 , a kind of automatic analysis method based on BSA gene localization shown in the figure, comprises the following steps:

[0023] (1) Prepare raw data for analysis;

[0024] (2) Detect whether the prepared file meets the conditions (file name and number of files), and judge whether it is satisfied. If it is satisfied, transfer the data to the corresponding working directory for the next step. If not, the corresponding prompt information will be output on the screen. The task corresponding to the project exits;

[0025] (3) If there is no problem in the above judgment, start to analyze the main process. If there is a problem in the process, the corresponding prompt information will be output for error correction.

[0026] In practical application, the toolkit that method of the present invention utilizes comprises 1 Shell script code altogether, and script name is as follows:

[0027] (a)BSA_Pipelines.sh

[0028] The code of this script is written based ...

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Abstract

The invention discloses an automated analysis method based on BSA gene positioning. The method comprises the following steps: step one, making original data preparation; step two, preparing data verification and inputting; and step three, carrying out data processing; and step four, carrying out result sorting. According to the Linux-shell-language-based automated analysis method, full-automatic analysis of the lower machine data is realized, so that the data processing efficiency and the server use efficiency are improved; and a gene region associated with the specific character is obtained finally and a rich positioning result display chart is provided.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to the technical field of Illumina sequencing data analysis, and specifically refers to an automatic analysis method based on BSA gene positioning. Background technique [0002] High-throughput sequencing technology provides more feasible solutions for people to use molecular biology methods to study the laws of nature. At present, many high-throughput sequencing technologies have relevant analysis methods or analysis processes as a reference to help researchers perform high-throughput data processing. [0003] Mixed grouping analysis (BSA) is a method of sequencing the DNA of multiple individuals with the same genetic background after mixing, and then calculating the variation frequency of the variation site (usually SNP) in the mixed DNA pool to locate the gene region associated with a specific trait Methods. The BSA method overcomes the limitation that it is diffic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/00G16B20/00
Inventor 王萌
Owner SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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