Minitype optical derivatization device for aflatoxin and sulfonamides

A technology of sulfonamides and aflatoxins, which is applied in the field of optical detection, can solve the problems of reduced light derivation efficiency, reduced band broadening, and short light source life, and achieves the goal of reducing the volume of the derivation pool, shortening the analysis time, and reducing production costs. Effect

Active Publication Date: 2019-02-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

But at present, whether it is a photochemical derivatizer for scientific research (Fan Haidong, Wang Lingfeng, Zhao Dongji, Self-made Photochemical Derivatization Reactor-High Performance Liquid Chromatography for the Determination of Aflatoxin B1 in Soybean Oil, Physical and Chemical Testing-Chemical Volume, 2013, 49, 999- 1001) or a commercial photochemical derivatizer (http://www.josvok.com/product_show.php?id=57), (http://www.18show.cn/zt410303/Product_10226818.html), the excitation light source They are all 254nm low-pressure mercury lamps with a lifespan of only 2000-3000 hours. The emitted light intensity gradually attenuates with the increase of the lighting time of the light source, and the light derivative efficiency decreases accordingly, resulting in a continu

Method used

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  • Minitype optical derivatization device for aflatoxin and sulfonamides
  • Minitype optical derivatization device for aflatoxin and sulfonamides

Examples

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Example Embodiment

[0034] Example 1

[0035] A post-column light derivatizer for high performance liquid chromatography (HPLC), the excitation light source is a high-power central wavelength 365nm ultraviolet LED, the bandwidth (FWHM) is 10nm, the divergence angle is 60°, and the rated current is 800mA; the derivation reaction tube is highly transparent The FEP tube has an inner diameter of 0.25 mm, an outer diameter of 1 / 16 inch, and a length of 18 cm. The volume of the derivation pool is 10 μL, and the distance between the derivation reaction tube and the LED light source is 7 mm; the derivation reaction tube is spirally wound on the inner surface of the reflective bowl and fixed with a thin steel wire; The LED substrate and heat sink are made of metal aluminum. The photochemical derivatizer was connected with Shimadzu RF-20A fluorescence detector to evaluate its performance. The liquid chromatography conditions are: chromatographic column: C18 column, 4.6mm×150mm×5μm; mobile phase A methanol...

Example Embodiment

[0039] Example 2

[0040] The photochemical derivatizer as described in Example 1 was combined with Shimadzu RF-20A fluorescence detector to analyze and detect 4 kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0041] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and RF-20A was not detected when the photochemical derivatizer was not connected; after the photochemical derivatizer was connected, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15.

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Abstract

The invention provides a minitype optical derivatization device for aflatoxin and sulfonamides. The minitype optical derivatization device comprises an ultraviolet LED light source with an excitationwavelength in a range of 280 to 370 nm, and adopts an ultraviolet-permeable pipe with a small internal diameter as a derivation reaction pool, wherein a derivatization reaction pipe is spirally woundon the inner wall of alight-reflective bowl in a single layer manner, and has an internal diameter of 0.10 to 0.35 mm and a length of 17 to 30 cm; and the derivation reaction pool has a volume of 7 to15 [mu]L. The optical derivatization device provided by the invention increases the fluorescence intensity of aflatoxin B1 by 5 times; and the optical derivatization device meets the requirements ofliquid chromatography post-column derivatization and flow injection analysis derivatization, and is used for analyzing trace aflatoxin and sulfonamides in a sample through combined application of high-performance liquid chromatography (HPLC) and ultra-high-pressure liquid chromatography (UPLC).

Description

technical field [0001] The patent of the present invention relates to the field of optical detection of high performance liquid chromatography (HPLC) and flow injection analysis (FIA), more specifically, relates to a light derivatizer for aflatoxin and sulfa drugs. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins by high-performance liquid chromatography (HPLC)-fluorescence detection, such as national standards GB / T 18979-2003 and GB / T 23212-2008, and the 2015 Chinese Pharmacopoeia that photoderivatization The instrument is included in the chapter on the determination of aflatoxin, which is used to assist in the determination of trace aflatoxin in medicinal materials, decoction pieces and preparations. Aflatoxin (AFTS) is a molecular mycotoxin, which is known as the strongest natural carcinogen. In 1993, aflatoxin was classified as a class I carcinogen by the Cancer Research Institute of the World Health Organization...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/74G01N21/64
CPCG01N21/643G01N30/02G01N30/74
Inventor 关亚风王楠耿旭辉宁海静冯春波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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