Yellow ginger cultivation method for increasing diosgenin content
A technology of diosgenin and cultivation method, which is applied in horticultural methods, botany equipment and methods, culture medium, etc., can solve problems such as restricting the application of anther cultivation technology, low anther cultivation efficiency, mixed ploidy of regenerated plants, etc., and achieves improvement Effects of callus induction rate, increase of diosgenin content, and improvement of culture efficiency
Inactive Publication Date: 2019-03-01
宋兆霞
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Problems solved by technology
However, there are not many domestic studies on the cultivation of turmeric anthers, and there are still problems of low anther culture efficiency and mixed ploidy of regenerated plants, which restricts the application of anther culture technology in the cultivation of new varieties of turmeric
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Embodiment 1
[0028] Beginning in 2015, test according to the yellow ginger cultivation method of the present invention, detailed steps are as follows:
[0029] 1) Selection of cultivation materials: Anjiang No. 3 planted in the Xunyang Yellow Ginger Planting Base in Shaanxi was selected as the cultivation material. Anjiang No. 3 is a new yellow ginger variety approved by the Shaanxi Provincial Forest Tree Variety Approval Committee. It has strong resistance and high yield , with high diosgenin content, it is a high-quality variety of turmeric; the male single plant of Anjiang No. 3, which grows vigorously and has no disease in the field, is selected to collect flower buds.
[0030] 2) Bud pre-treatment and surface disinfection: During the full flowering stage of Anjiang 3, the flower buds on a single male plant were collected before 10:00 a.m., and the flower buds at the single-nucleate stage of microspore development were screened out (the screening method was: Pick the anthers in the flo...
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The invention provides a yellow ginger cultivation method for increasing diosgenin content. The method comprises the following steps: 1) optimally selecting cultivation materials; 2) pretreating flower buds and performing surface sterilization; 3) inoculating and inducing anther; 4) performing callus differentiation; 5) rooting seedlings; 6) identifying plant ploidy and screening diploid anther regeneration plants; 7) planting in field after domestication; 8) hybridizing super male plants and female plants of yellow ginger, thereby acquiring new male strains. According to the invention, an anther culture technology is firstly applied to yellow ginger cultivation and the diploid anther regeneration plants of yellow ginger are successfully acquired; the super male plants and female plants inthe diploid anther regeneration plants are hybridized, so that the new strain of yellow ginger with pure male descendants can be acquired; the diosgenin content in male yellow ginger plants is higherthan that of female plants, so that the new strain of yellow ginger cultivated according to the invention can greatly increase the diosgenin content and can effectively guarantee the sustainable development of yellow ginger industry.
Description
technical field [0001] The invention relates to the field of medicinal plant cultivation, in particular to a yellow ginger cultivation method for increasing the content of diosgenin. Background technique [0002] Turmeric, scientific name is Dioscorea shield leaf ( Dioscorea zingiberensis C.H.Wright), also known as fire vine root and fire head root, belongs to the rhizome group of Dioscorea genus in Dioscoreaceae. It is a perennial winding herbaceous vine. Its morphological characteristics are dioecious, rhizomes grow horizontally, leaves are thick and papery, and single leaves are alternate. , triangular ovate or long ovate, with petiole shield-shaped growth, hence the name Dioscorea shield leaf, which is the main feature that distinguishes it from other species of Dioscorea genus. Turmeric is a unique species in my country and also the main medicinal plant in my country. It is mainly distributed in Henan, Hubei, Hunan, Shaanxi, Gansu, Sichuan and other regions. In the wi...
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IPC IPC(8): A01H4/00A01G22/25A01G24/15A01G24/28A01G24/10A01G24/25A01G24/30A01H1/02
CPCA01H1/02A01H4/001A01H4/008A01G22/25A01G24/10A01G24/15A01G24/25A01G24/28A01G24/30
Inventor 宋兆霞
Owner 宋兆霞