A kind of active microalgae nutrient restoration liquid and preparation method thereof

A technology for repairing liquid and microalgae, applied in the agricultural field, can solve problems such as limiting the value of fertilizers, and achieve the effects of enhancing stress resistance, vigorous vitality, and reducing absorption

Active Publication Date: 2020-03-13
雷云飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microalgae added to biofertilizers are usually dead and can only use the nutrients in their own tissues, which greatly limits their value as fertilizers

Method used

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  • A kind of active microalgae nutrient restoration liquid and preparation method thereof
  • A kind of active microalgae nutrient restoration liquid and preparation method thereof
  • A kind of active microalgae nutrient restoration liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of active microalgae nutrient restoration liquid, the preparation method comprises the following steps:

[0042] S1, expanding the cultivation of the green algae Chlorella pyrenoidosa and the nitrogen-fixing cyanobacteria Anabaena anabaena, respectively, to obtain two types of algae liquids, among which, in the process of expanding cultivation,

[0043] The expanded cultivation process of the two algae liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15 mL of medium solution for 10 days to obtain 15 mL of algal liquid → transfer to 85 mL of medium solution Continue culturing for 10 days to obtain 100mL of algae solution → inoculate into 0.9L medium solution for 10 days to obtain 1L of algae solution → inoculate into 4L medium solution for 10 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 10 days to obtain 18L of algae liquid → transfe...

Embodiment 2

[0048] The difference from Example 1 is that in the expanded cultivation of step S1,

[0049] The expanded cultivation process of the two algae solutions includes: selecting and separating a single microalgae cell under a microscope → culturing a single microalgae cell in 15mL of medium solution for 12 days to obtain 15mL of algae solution → transfer to 85mL of medium solution Continue culturing for 10 days to obtain 100mL of algae liquid → inoculate into 0.9L medium solution for 12 days to obtain 1L of algae liquid → inoculate into 4L medium solution for 12 days to obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 12 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 12 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in the final obtained algae liquid...

Embodiment 3

[0052] The difference from Example 1 is that in the expanded cultivation of step S1,

[0053] The expanded cultivation process of the two algal liquids includes: selecting and separating a single microalgal cell under a microscope → culturing a single microalgal cell in 15 mL of medium solution for 15 days to obtain 15 mL of algal liquid → transfer to 85 mL of medium solution Continue culturing for 15 days, obtain 100mL of algae liquid → inoculate into 0.9L medium solution and continue culturing for 15 days, obtain 1L of algae liquid → transfer and inoculate into 4L medium solution for 15 days, obtain 5L Algae liquid → inoculated into 13L medium solution and continued to cultivate for 15 days to obtain 18L of algae liquid → transferred to 782L medium solution and continued to cultivate for 15 days to obtain 800L of algae liquid. At the end of the expansion of each stage above, the number of viable microalgae cells is ≥10 6 cells / mL, the number of active microalgae cells in th...

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Abstract

The invention discloses an active micro-algae nutritional restoration solution, which belongs to the technical field of agriculture. The active micro-algae nutritional restoration solution is characterized by comprising multiple micro-algae at a living state, wherein the micro-algae comprises chlorella pyrenoidosa and anabaena azotica; by calculating according to the quantity of micro-algae cells,a ratio of the chlorella pyrenoidosa to the anabaena azotica is 1: (0.2 to 5). The invention also discloses a preparation method of the active micro-algae nutritional restoration solution. The preparation method of the active micro-algae nutritional restoration solution comprises the following steps: S1, separately mass cultivating the chlorella pyrenoidosa and anabaena azotica, and obtaining twoalgae solutions; and S2, mixing the two algae solutions, and obtaining the active micro-algae nutritional restoration solution. The active micro-algae nutritional restoration solution has the effectsof restoring the soil, supplementing the natural nitrogen element, activating the soil trace elements, improving the stress resistance of plants, preventing diseases and insects, and improving the quality and yield of agricultural products.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to an active microalgae nutrient restoration liquid and a preparation method thereof. Background technique [0002] When the soil fertility level cannot meet the nutrients required for plant growth, the soil needs to be fertilized. Chemical fertilizers are currently widely used fertilizers. However, the negative effects of chemical fertilizers cannot be ignored: the raw materials for the production of chemical fertilizers contain Zn, Heavy metals such as Cu, Co, and Cr, these heavy metals are applied to the soil with chemical fertilizers, and cause the accumulation of heavy metal elements in the soil, which are absorbed into the plants through plants, affecting plant growth and the quality of agricultural products; chemical fertilizers will reduce soil microorganisms The quantity and activity of soil microorganisms, thereby reducing the transformation of organic matter in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C05G3/60C05G3/80C12R1/89
CPCC05F11/08C05G3/00C05G3/80C05G5/20C12N1/12C12N1/20
Inventor 雷云飞
Owner 雷云飞
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