A screening method for single-target gene editing t0 generation tobacco strains

Abstract

The invention discloses a method for screening single-target gene editing T0 generation tobacco strains, comprising: (1) using a handheld computer to collect sample information and wirelessly connecting with a server terminal; Manage the tobacco leaf samples, and complete the screening of tobacco strains that need to be collected in real time, the collection of tobacco leaf samples, receipt, processing, storage, sample distribution, testing, storage of surplus samples and disposal of surplus samples; and The test results are fed back to the tobacco plant growers, so as to complete the screening of single-target gene-edited T0 generation tobacco strains; the T0 generation tobacco strains are gene-edited regenerated seedling tobacco strains. The method of the present invention can achieve efficient and accurate screening of a large number of similar single-target gene edited T0 generation tobacco strains.

Description

technical field [0001] The present invention relates to the field of computer software, in particular to a method for screening a large number of tobacco strains for efficient and accurate single-target gene editing T0 generation in gene editing breeding. Background technique [0002] Gene editing breeding technology has been widely used in the genome modification of various plants, such as Arabidopsis, rice and tobacco. With the establishment of tobacco gene editing and breeding factories and the rapid advancement of material creation, a large number of gene editing materials have been created. These editing materials have become the focus of attention in the screening of tobacco plant phenotypes. There are differences in biochemical indicators. Therefore, how to efficiently and accurately screen tobacco strains from massive editing materials has become an urgent problem to be solved. [0003] Since the screening of tobacco strains involves multiple steps such as tobacco ...

AI Technical Summary

Problems solved by technology

[0003] Since the screening of tobacco strains involves multiple steps such as tobacco strain selection, tobacco leaf sample collection, processing and analysis feedback, including tobacco strain selection that requires tobacco leaf sample collection, tobacco strain sampling marking, special phenotype recording, tobacco leaf collection and bagging, and tobacco leaf freezing , freeze-drying of tobacco leaves, grinding of tobacco leaves, storage of tobacco leaf powder, testing and processing of residual samples, and finally re-screening of massive tobacco strains according to the feedback of the test results of tobacco leaves. Traditionally, manual use of a marker pen is used to copy the code of the tobacco plant to the sample bag or sample tube, and then gradually enter the code into the computer terminal. These operations require high personnel quality and are prone to various errors, which seriously affect the sample management process. Efficient and accurate screening of editing materials affecting tobacco leaves

At the same time, there is a large randomness in the sampling and pre-processing process. If there is no unified management, it will bring instability to the subsequent detection.

These have seriously affected the establishment of tobacco gene editing breeding factories and the rapid advancement of material creation