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Solution and method for detecting body activity

A technology of activity and solution, applied in the fields of biology, veterinary medicine and medical research, can solve problems such as large fluctuations and uncertainties, achieve high accuracy, avoid waste, and save costs

Inactive Publication Date: 2019-03-05
梁肆生
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  • Summary
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Problems solved by technology

However, the activity of enzymes in saliva is greatly affected by the state of saliva secretion, and the activity of enzymes is also fluctuated by the influence of pH value of saliva, minerals and food residues in saliva, so the determination of enzyme activity in saliva has a strong Therefore, how to measure the activity of enzymes in saliva, and then evaluate the state of the body has important clinical guiding significance

Method used

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Examples

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Effect test

Embodiment 1

[0042] A solution for detecting body activity, which is prepared by the following components:

[0043] Tris-HCl pH 7.6: 60-150mM;

[0044] Glucocorticoid: 220IU / L;

[0045] 2. 4, 6-Triiodo-3-hydroxybenzoic acid: 0.07%;

[0046] 4-aminoantipyrine: 0.07%;

[0047] Glutathione: 1100IU / L.

[0048] The method of using this solution to detect body activity includes the following steps:

[0049] 1) Collect saliva of ten people separately;

[0050] 2). Mix the saliva of ten people with the solution; wherein the mixing volume ratio of the saliva to the solution is 1:15;

[0051] 3) Determine the activity of the organism according to the color depth of the mixture obtained after mixing the saliva and the solution; the darker the blue, the stronger the resistance and the healthier the body; the darker the blue, the weaker the resistance and the weaker the body. As shown in Table 1, the results of glucose metabolism of ten people, the numbers 1-10 respectively represent the 10 people selected, among w...

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Abstract

The invention discloses a solution and method for detecting body activity, and the solution is prepared from 60-150 mM of Tris-HCl with pH of 7.6; 0.12-0.4% of chromogen; 1200-2500 IU / L of glucose oxidase; 120-250 IU / L of horseradish peroxidase and 600-1500 IU / L of an antioxidant. An evaluation method comprises the following steps: 1) collecting saliva; 2) mixing the saliva with the solution; and 3), determining the glucose metabolism level of a body according to the color change of a mixed solution obtained after the saliva and the solution are mixed. According to the method disclosed by the invention, the body activity is known by adopting the solution which generates color change after being reacted with the saliva, scientificity and quantitative problems of body health evaluation aresolved, and in addition, the detection method is visual, convenient and rapid, high in efficiency and high in accuracy. At the same time, the antioxidant is added to the solution to extend the service life of the solution, waste is avoided, and cost is saved.

Description

Technical field [0001] The invention relates to the technical fields of biology, veterinary medicine and medical research, in particular to a solution and a detection method for detecting body activity. Background technique [0002] The health of the body can be measured through the health evaluation system, but it needs to go through a complicated blood sampling and testing process. Blood sampling itself is also a kind of harm to the body. [0003] Scientific research shows that saliva can be used for the detection of human antibodies, the detection of pathogens, the detection of biochemical indicators such as body activity, coldness, sugar content, uric acid and creatine. Saliva is an important fluid secreted by the body’s oral glands (parotid, submandibular, and sublingual glands). Its composition changes with the change of the body’s health status. In particular, the enzyme concentration and enzyme activity in the saliva are important for changes in the body’s vitality status. ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 不公告发明人
Owner 梁肆生
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