Tree moss soft pseudo-carbonized fermented granules and preparation method and application thereof
A particle and tree moss technology is applied in the field of cigarette material preparation, which can solve the problems of inability to directly flavor cigarettes with harmonization of aroma, poor adsorption effect, and insignificant adsorption effect of adsorbing material particles, achieves good application value, improves aroma and reduces coke, the effect of convenient industrial production
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Embodiment 1
[0035] 1. Soft carbonization of tree moss
[0036] Put the moss into the carbonization furnace, gradually raise the furnace temperature to about 80°C, and keep it for 40 minutes. After the moss is dry, quickly raise the temperature to 120°C, and keep it for 3 hours. Turn off the heat source, and take out the soft carbonized sample of the moss for use. Generally, the raw material turns taupe, and the carbonization is completed when the weight is 50-80% of that before the carbonization.
[0037] 2. Cultivation of Bacillus subtilis Van3 (B.subtilisVan3)
[0038] A. Test tube slant culture
[0039]A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, incubate at 28°C for 24 hours to obtain test tube seeds, transfer the liquid strains to 1L of LB liquid medium according to the inocu...
Embodiment 2
[0045] 1. Soft carbonization of tree moss
[0046] Put the moss into the carbonization furnace, gradually raise the furnace temperature to 80°C, and keep it for 30 minutes. After the moss is dry, quickly raise the temperature to 140°C, and keep it for 2.5 hours.
[0047] 2. Cultivation of Bacillus subtilis Van3
[0048] B. Seed culture
[0049] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate Van35 test tube species, cultivate at 28°C for 24h to obtain liquid strains, transfer the liquid strains to 1L of LB liquid medium at 5% (volume percentage of liquid strains, v / v), 37°C, 150r Centrifuge the culture solution at 3500 r / min for 10 min, wash the precipitate with sterile water, centrifuge, and finally shake evenly with 20 mL of sterile water, and dilute it 10 times before use, which is the Bacillus subtilis Van3. ...
Embodiment 3
[0054] Example 3: Moss Soft Pseudo-Carbonization Fermentation Granules Applied in Cigarette Filter Sticks
[0055] The moss flexible pseudo-carbonization fermentation granules obtained in Example 1 are applied to cigarette composite cigarette filter sticks, and 1.5 mg of granular material is added per millimeter of standard cigarette filter sticks to prepare experimental cigarettes, which are named No. 1 cigarettes; Cigarettes without the above-mentioned particles were used as the control sample, and the moisture content of the cigarette samples was adjusted to 12wt% according to the national standard "GB / T16447-1996 Atmospheric Environment for the Regulation and Testing of Tobacco and Tobacco Products", and the smoking expert group was evaluated according to "GB5606.4- 1996 Cigarette Sensory Technical Requirements" to carry out sensory evaluation of cigarettes and detection of harmful components in smoke. See Table 1 for the evaluation results; see Table 2 and Table 3 for spe...
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