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Tissue culture method of coconut mature zygotic embryos

A tissue culture and zygotic embryo technology, applied in the field of plant tissue culture, can solve the problems of poor test repeatability, no success, low reproduction coefficient, etc., and achieve the effect of improving callus induction rate, shortening tissue culture period, and good repeatability.

Active Publication Date: 2019-03-19
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Coconut is a highly heterozygous plant, and none of the culture media and cultivation methods published so far is widely applicable, and they are all limited to a certain type of genotype, or even in the same variety, some Plants can successfully induce callus, while some cannot
The Coconut Research Institute in Sri Lanka has a specialized tissue culture center, and they have been facing this problem for decades, which is difficult to overcome
[0006] 2. Poor test repeatability
The regenerated plants obtained by the Philippine Coconut Agency for the first time have already set fruit, but subsequent experiments have not been successful
[0008] 3. The tissue culture cycle is long
It will take longer to establish a mature technology system that can be used on a large scale
[0010] In addition, problems such as low reproductive coefficient and difficult reproduction need to be solved urgently.
[0011] In summary, there is currently no mature technical solution available for production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A kind of mature zygotic embryo tissue culture method of coconut, comprises the following steps:

[0057] The mature zygotic embryos of Wenye No. 2 were taken as explants; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant rooting stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0058] 1.1 Callus induction stage:

[0059] The callus induction medium includes the following components: 110 μM 2,4-D, 8.8 μM 6-BA, 4 g / L phytogel, 3 g / L activated carbon and 40 g / L sucrose are added to the Y3 medium;

[0060] The pH value of the medium is 5.75, the culture condition is dark culture at 28°C, and the culture time is 1 month.

[0061] 1.2 Stage of callus propagation:

[0062] The callus propagation medium is the same as the callus induction medium;

[0063] The pH value of the medium was 5.75, and the culture c...

Embodiment 2

[0076] A kind of mature zygotic embryo tissue culture method of coconut, comprises the following steps:

[0077] The mature zygotic embryos of Wenye No. 2 were taken as explants; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant rooting stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0078] 1.1 Callus induction stage:

[0079] The callus induction medium includes the following components: 100 μM 2,4-D, 5 μM 6-BA, 2 g / L phytogel, 1 g / L activated carbon and 20 g / L sucrose are added to the Y3 medium;

[0080] The pH value of the medium is 5.75, the culture condition is dark culture at 27°C, and the culture time is 1 month.

[0081] 1.2 Stage of callus propagation:

[0082] The callus propagation medium is the same as the callus induction medium;

[0083] The pH value of the medium was 5.75, and the culture con...

Embodiment 3

[0097] A kind of mature zygotic embryo tissue culture method of coconut, comprises the following steps:

[0098] The mature zygotic embryos of Wenye No. 2 were taken as explants; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant rooting stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0099] 1.1 Callus induction stage:

[0100] The callus induction medium includes the following components: 150 μM 2,4-D, 10 μM 6-BA, 8 g / L phytogel, 8 g / L activated carbon and 50 g / L sucrose are added to the Y3 medium;

[0101] The pH value of the medium is 5.80, the culture condition is dark culture at 29°C, and the culture time is 3 months.

[0102] 1.2 Stage of callus propagation:

[0103] The callus propagation medium is the same as the callus induction medium;

[0104] The pH value of the medium was 5.80, and the culture con...

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PUM

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Abstract

The invention discloses a tissue culture method of coconut mature zygotic embryos. The coconut mature zygotic embryos are used as explants; a culture medium formula is strictly controlled at each phase of tissue culture; and after the tissue culture is carried out, the callus induction rate can reach 98.0 percent, the highest embryo induction rate can reach 98.4 percent, the highest embryo regeneration rate can reach 98.7 percent and the highest transplanting survival rate can reach 98.1 percent. The culture medium formula and the method, disclosed by the invention has a board spectrum, is applicable to various varieties including Hainan local high-height seeds, Wenye No. 2, Wenye No. 3 and the like, and has good repeatability and a short period; and industrialized and commercial popularization and application are facilitated.

Description

technical field [0001] The invention relates to a tissue culture method of coconut mature zygote embryo, belonging to the technical field of plant tissue culture. Background technique [0002] Tissue culture technology is a method of rapidly propagating clones using explants such as roots, stems, leaves, flowers, and fruits of plants, and has been successful in various plants. Tissue culture technique is the most effective vegetative method for rapid propagation of coconuts. In terms of coconut tissue culture, researchers have successively used zygotic embryos, immature inflorescences, young ovaries, stem tips, young leaves, etc. for in vitro culture, all of which have been successful to varying degrees. As early as 1978, Fisher and Tsai attempted to use coconut embryos for tissue regeneration, but only obtained callus tissue and failed to regenerate plants. In 1994, Verdeil et al. successfully obtained regenerated plants from immature inflorescences of coconut for the fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘蕊范海阔
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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