Kit for specifically detecting abdominal obesity through detecting rs12409479

A technology of abdominal obesity and a kit, which is applied in the field of SNP typing detection, can solve the problems of low accuracy, cumbersome operation, and long time consumption, and achieve the effect of good accuracy, simple steps, and high specificity

Inactive Publication Date: 2019-03-22
刘露
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, this method has disadvantages such as time-consuming, cumbersome operation, and low accuracy.
There are also PCR combined with DNA sequencing methods for the detection of gene polymorphism sites, but this method is limited in the application of large-scale population screening or detection of multiple genes and multiple sites

Method used

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  • Kit for specifically detecting abdominal obesity through detecting rs12409479

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Obtaining of SNP molecular markers

[0020] sample selection

[0021] By applying for download from the National Institutes of Health and cooperating with the research group of Professor Deng Hongwen, an expert in osteoporosis research, the following six samples with fat content measurement were obtained:

[0022] (1) Framingham Heart Study (FHS): FHS is a longitudinal prospective family cohort study involving more than 16,000 people from three generations. The first generation consisted of 5,209 residents living in the small town of Framingham in Massachusetts, USA. The second generation is the adult children of the first generation and their spouses, totaling 5124 people. The third generation is the grandchildren of the first generation, with a total of more than 4,000 people. First-generation participants underwent dual-energy X-ray (DXA) absorptiometry at visits 22 to 24. This instrument not only measures bone density, but also accurately measures body...

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PUM

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Abstract

The invention discloses a kit for specifically detecting abdominal obesity through detecting rs12409479. The kit comprises a specific primer pair and a specific fluorescent probe pair which are used for detecting a No. rs12409479 SNP (Single Nucleotide Polymorphism) site, a conventional assembly for carrying out fluorescent quantitative PCR (Polymerase Chain Reaction) detection and the like. The kit disclosed by the invention is used for evaluating the hereditary susceptibility of the abdominal obesity of individuals through detecting a single nucleotide polymorphism site gene type on the rs12409479 which is tightly related to the hereditary susceptibility of the abdominal obesity at the same time.

Description

technical field [0001] The invention relates to the field of SNP typing detection in the technical field of genetic engineering, in particular to a kit for specifically detecting abdominal obesity by detecting rs12409479. Background technique [0002] Obesity is a chronic metabolic disease characterized by increased body fat stores. It is a serious public health problem that can significantly increase the risk of type 2 diabetes, hypertension, cardiovascular disease, and cancer. It has become one of the leading causes of disability and death worldwide. More than two-thirds of adults in the United States are overweight, and more than one-third are obese. Annual economic losses related to obesity exceed $200 billion. [0003] Obesity is a major killer that currently threatens human health. The chief executive of the British National Health Service System (NHS) pointed out that successfully losing 5% to 7% of body weight can reduce the risk of diabetes by 60%. In order to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 刘露
Owner 刘露
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