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A method for accelerating the accumulation of Paris polyphylla saponin I and Paris polyphylla saponin II

A technology of Parisiana saponin and Parisiana saponin, which is applied in the field of accelerating the accumulation of Parisiana saponin I and Parisiana saponin II when the concentration of carbon dioxide increases

Active Publication Date: 2021-04-30
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, in the prior art, there has been no improvement of CO in the culture environment. 2 concentration to increase the content of papaya saponin I and papaya saponin II

Method used

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  • A method for accelerating the accumulation of Paris polyphylla saponin I and Paris polyphylla saponin II

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Experimental materials and methods

[0024] (1) Pre-cultivation conditions of Paronymus diangi

[0025] Wash and weigh 40 annual plants of Prunus diangi, transplant them into seedling bags, and pre-cultivate them in a culture room for 10 days under normal atmospheric carbon dioxide concentration (400ppm). The light intensity is 300 μmol m -2 the s -1 , 12 hours of light per day, the cultivation temperature is 18-23°C, and the air humidity is 60%.

[0026] (2) Experimental culture conditions

[0027] After pre-cultivation for 10 days, 20 of them were placed in CO 2 Concentration of 800ppm in the cultivation room, the remaining 20 plants of Polyaceae were left in CO 2 Concentration of 400ppm (control) culture room, start to do a comparative test. In addition to CO 2 Except for the different concentrations, the other environmental culture conditions of the two culture chambers are the same. The light intensity is 300 μmol m -2 the s -1 , 12 hours of light per ...

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Abstract

The present invention provides a method for accelerating the accumulation of S. polyphylla saponin I and S. polyphylla saponin II. The method includes increasing the concentration of carbon dioxide in the environment and accumulating the contents of S. Building pre-culture, carbon dioxide concentration treatment culture steps. The invention belongs to the technical field of bioengineering and is carbon dioxide (CO 2 ) Concentration increases to promote a new pathway for the synthesis of papaya saponin I and papaya saponin II. CO in the environment 2 The increase of concentration accelerated the accumulation of sarcoside I and sarcoside II in S. dianthus seedlings. This has a role in promoting production in actual production. By changing the content of the main carbon source required by the plants in the cultivation environment, the demand for increasing the production of Parisia diarrhea in actual production can be met, and the actual production efficiency and the utilization efficiency of a single plant of Parisia diarrhea can be further improved.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, and specifically relates to carbon dioxide promoting the synthesis of pachyphyll saponin I and papaya saponin II. methods and applications. Background technique: [0002] carbon dioxide (CO 2 ) participates in the main metabolic activities in plants, and is an indispensable raw material for the synthesis of organic matter for plants. According to the latest data, in November 2018, atmospheric CO 2 The concentration has reached 408ppm, and CO in the atmosphere 2 concentration is still rising. However, for some plants, an appropriate increase in CO in the growth environment 2 Concentration, can promote the growth of plants. Some literatures reported that at high concentrations of CO 2 Under environmental conditions, some secondary metabolites in plants will also accelerate accumulation. For example, at high concentrations of CO 2 Under this condition, lignin in plantain will accele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/02A01G22/25
CPCA01G7/02A01G22/25
Inventor 林亮贾艳霞强奇李唯奇高彦粉郁步竹王沐兰
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI