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A preparation method of protein-based nanoparticles of solubilization and synergistic insoluble bioactive substances

A bioactive substance and nanoparticle technology, which is applied in the field of functional food preparation, can solve the problems of the use of cross-linking agents and low loading, and achieve the effects of increasing the loading, reducing the dissolution rate, and controlling the operation.

Active Publication Date: 2021-09-21
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing preparation methods of protein-based nanocarriers (nanocomposite, emulsification-evaporation, covalent cross-linking, etc.) still have problems such as low loading capacity, use of cross-linking agents, and sudden release of drugs. The carrier preparation method with low cost, high drug loading, good stability and low biological toxicity is the most urgent problem to be solved in the field of food and medicine.

Method used

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  • A preparation method of protein-based nanoparticles of solubilization and synergistic insoluble bioactive substances
  • A preparation method of protein-based nanoparticles of solubilization and synergistic insoluble bioactive substances
  • A preparation method of protein-based nanoparticles of solubilization and synergistic insoluble bioactive substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Accurately weigh 5g of soybean 7S protein powder, disperse it in 100g of distilled water, and continuously stir it at room temperature for 2 hours, then place it in a refrigerator at 4°C for overnight hydration (add 1 drop to the protein solution with a mass concentration of 0.08 % sodium azide, to prevent the growth of microorganisms), to obtain a mass concentration of 5wt% of the original soybean 7S protein stock solution.

[0035] (2) Measure 3mL, 4mL, 5mL, 6mL, 7mL of absolute ethanol in 10mL serum bottles, mark as samples 1, 2, 3, 4, 5, weigh 15mg of curcumin powder and add to 5 samples respectively , and stirred for 1 h in the dark to obtain curcumin ethanol solution.

[0036] (3) Add 5mL, 4mL, 3mL, 2mL, and 1mL deionized water to samples No. 1, 2, 3, 4, and 5 respectively, stir well, and prepare 8 mL of curcumin ethanol / water solutions with different ethanol volume fractions.

[0037] (4) In the sample of step (3), add 2 mL of original soybean 7S protein sto...

Embodiment 2

[0041] According to the method described in Example 1, a whey protein-curcumin assembly nanoparticle solution was prepared. Except that soybean 7S protein was replaced by whey protein freeze-dried sample, and the protein dispersion was hydrated overnight and then centrifuged (8000g, 20min), other operations were the same as in Example 1. After the whey protein-curcumin assembly particle solution is prepared, take 0.1mL sample in 3mL ethyl acetate, vortex for 60 seconds, let it stand for 30min, take the supernatant according to the ethyl acetate-curcumin standard curve to measure its content Encapsulation efficiency, in the present embodiment, 50% ethanol-treated whey protein has the highest encapsulation efficiency, which can reach 86.3%, that is, 129 mg of curcumin can be buried per gram of whey protein.

Embodiment 3

[0043] According to the method described in Example 1, a bovine serum albumin-curcumin assembly nanoparticle solution was prepared. Except that the soybean 7S protein was replaced with a bovine serum albumin sample, other operations were the same as in Example 1. After the bovine serum albumin-curcumin assembly particle solution is prepared, take a 0.1mL sample to measure its encapsulation efficiency. In this embodiment, the bovine serum albumin treated with 40% ethanol has the highest encapsulation efficiency, which can reach 95.1%. That is, 143 mg of curcumin can be embedded per gram of bovine serum albumin. The reason why bovine serum albumin has such a high encapsulation efficiency is that bovine serum albumin contains a large number of disulfide bonds. Ethanol denatures bovine serum albumin and expands its structure. The sulfur bond is then opened, and when the ethanol is removed by dialysis, in addition to the traction force of the non-covalent bond, there is also the tr...

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Abstract

The invention belongs to the technical field of functional food preparation, and discloses a method for preparing protein-based nano-particles of bioactive substances that promote dissolution and synergize insoluble bioactive substances. Add protein powder into water, hydrate after stirring and dispersing to obtain a protein solution; dissolve insoluble active substances in a polar organic solvent, then add the obtained protein solution and stir to disperse evenly to obtain a mixed solution; dialyze the mixed solution to remove the polar organic solvent Solvent, centrifuged to remove insoluble matter, to obtain protein-based nanoparticle solution. The method of the present invention is simple in process, and the obtained nano-products can effectively enhance the water solubility and physical and chemical stability of insoluble active substances, and can effectively enhance the biological potency of active substances, and have broad application in food, health products, daily chemical products and pharmaceutical industries. application space.

Description

technical field [0001] The invention belongs to the technical field of functional food preparation, and in particular relates to a method for preparing protein-based nanoparticles of bioactive substances that promote dissolution and synergize insoluble bioactive substances. Background technique [0002] A number of studies have confirmed that reasonable supplementation of some biological factors with specific functions can prevent and delay the occurrence of tumors and cardiovascular diseases. Therefore, the development of some functional foods is an inherent demand for the development of the health industry. The development of functional food needs to break the shackles of the solubility, stability and low bioabsorption rate of functional factors. Many patents use emulsion (201510169051.1), particles (201610178852.9), liposome (201310413928.8), micelles (201010120171.X) and other systems to improve the bioavailability of insoluble substances, but in the process of preparing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/06A23J1/08A23J1/14A23J1/20A23J3/04A23J3/08A23J3/12A23J3/16A61K9/51A61K9/52A61K47/42A61K31/12
CPCA23J1/06A23J1/08A23J1/14A23J1/205A23J3/04A23J3/08A23J3/12A23J3/16A23V2002/00A61K9/5169A61K31/12A23V2250/54252A23V2250/5428A23V2250/5436A23V2250/5488
Inventor 唐传核刘玲玲
Owner SOUTH CHINA UNIV OF TECH
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