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Tissue culture propagation method of Osmanthus chinensis induced by young sporangia group through ggb pathway

A technology for sporangia and E. japonica, which is applied in the field of tissue culture and reproduction of E. chinensis in South China, can solve the problems of difficulty in obtaining explants and difficulty in induction, and achieve the effects of high induction rate, short induction time, and high environmental requirements.

Active Publication Date: 2021-08-24
XUZHOU VOCATIONAL COLLEGE OF BIOENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, the bottleneck of fern tissue culture research is mainly concentrated in the following two aspects: first, it is difficult for most ferns to obtain suitable explants directly from live plants planted in the ground, and it is extremely difficult to induce; The induction of green globules is the key technology when the green globular body (Green Globular Body, GGB) approach is used for tissue culture propagation (explant-green globular body-plant)

Method used

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  • Tissue culture propagation method of Osmanthus chinensis induced by young sporangia group through ggb pathway
  • Tissue culture propagation method of Osmanthus chinensis induced by young sporangia group through ggb pathway
  • Tissue culture propagation method of Osmanthus chinensis induced by young sporangia group through ggb pathway

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Selection of explants and surface disinfection: Using the high-quality Osmanthus chinensis seedlings successfully introduced and cultivated in the school base as materials, on April 20, 2018, healthy incomplete sporangia with young sporangia were cut. Unfold the leaves, gently remove surface dirt with a soft brush, rinse with running water, soak in 75% ethanol for 20 s, then wash with 0.1% mercuric chloride for 4 min, rinse with sterile water 7 times, and obtain Osmanthus chinensis without water. Sporangia explants of young leaves of fungi;

[0030] (2) Induction of green spheroids: The aseptic young leaf sporangia explants of Osmanthus chinensis obtained in step (1) were excised end to end, cut into 2 cm segments, and carefully inoculated into GGB induction medium according to the direction of polarity. The medium is B5 basic medium, with 30 g / L sucrose and 7 g / L agar added, and the pH is 5.8. The number of inoculations is 30 bottles, with 1 explant in each bottle...

Embodiment 2

[0037] (1) Selection of explants and surface disinfection: On June 7, 2018, the high-quality Osmanthus chinensis seedlings successfully introduced and cultivated in the school base were used as materials, and healthy incomplete sporangia with young sporangia were cut. Spread the leaves, gently remove surface dirt with a soft brush, rinse with running water, soak in 75% ethanol for 20 s, then shake and wash with 0.1% mercuric chloride for 3 min, rinse with sterile water 5-7 times, get Huanan Osmanthus aseptic young leaf sporangia explants;

[0038] (2) Induction of green spheroids: The aseptic young leaf sporangia explants of Osmanthus chinensis obtained in step (1) were excised end to end, cut into 2 cm segments, and carefully inoculated into GGB induction medium according to the direction of polarity. The composition of the medium is B5+6-BA1.0 mg / L+NAA0.1 mg / L, 30 g / L sucrose and 7 g / L agar are added, and the pH is 5.8. The number of inoculations and culture conditions are ...

Embodiment 3

[0045] (1) Selection of explants and surface disinfection: On July 4, 2018, the high-quality Osmanthus chinensis seedlings successfully introduced and cultivated in the school base were used as materials, and healthy incomplete explants with young sporangia were cut. Spread the leaves, gently remove surface dirt with a soft brush, rinse with running water, soak in 75% ethanol for 20 s, then wash with 0.1% mercuric chloride for 2 min, rinse with sterile water 5-7 times, get Huanan Osmanthus aseptic young leaf sporangia explants;

[0046] (2) Induction of green spheroids: the inoculation method, culture medium, inoculation quantity and culture conditions are the same as in Example 2, and will not be repeated here.

[0047] (3) Proliferation of green spheroids: On July 10, 2018, it was observed that there were many green spores scattered from the sporangia on the surface of the medium. On July 22, it was observed that the spores gradually expanded to form green spheroids of Osman...

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Abstract

The tissue culture propagation method of Osmanthus chinensis by the GGB pathway induced by young sporangia groups, including the selection of explants and surface disinfection, the induction of green spheroids, the proliferation of green spheroids, the differentiation of green spheroids, and the growth of differentiated seedlings The steps of seedling and rooting culture, seedling hardening and transplanting of Osmanthus chinensis tissue culture seedlings provide methods and approaches for breeding high-quality commercial seedlings of Osmanthus chinensis through tissue culture, and successfully established a plant with incompletely expanded leaves of Osmanthus chinensis. The young sporangia are explant tissue culture propagation method, and the South China Osmanthus chinensis seedlings are induced by the GGB method. The induction time is short, the induction rate is high, and it can be produced every year without seasonal restrictions, which solves the problem of conventional spore propagation. The environmental requirements are high, and the collection of spores is limited.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and propagation of Osmanthus chinensis through the GGB approach induced by young sporangia. Background technique [0002] South China purple Osmanthus ( Osmunda vachellii Hook.), a fern of the genus Osmundaceae of the order Osmundaceae, produced in the humid subtropical region of Asia, often wild in mountains, grasses or streams. This species has the characteristics of both Pachysystis and Thyscystis. It is the most primitive group of Physsina and is also one of the few existing transitional types between Pachysys and Thyss. [0003] Osmunda chinensis is a terrestrial fern with a plant height of 1-2m and thick rhizomes forming a cylindrical spindle. Leaves clustered at the top, thick papery, glabrous on both sides, yellow-green, slightly glossy; petiole 15-35 cm long, hard; leaves oblong, 30-80 cm long, 15-30 cm wide; pinnae short Stem,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陶佩琳高政平
Owner XUZHOU VOCATIONAL COLLEGE OF BIOENG
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