Blueberry anti-cancer instant drink

[0055] Example 1

[0056] The raw materials used to prepare the blueberry anti-cancer drink are: 60wt% of blueberry extract, 5wt% of seabuckthorn, 5wt% of medlar, 10wt% of dandelion whole plant, 10wt% of citrus mushroom, and 10wt% of lily.

[0057] The processing method of the blueberry anti-cancer brewed drink comprises the following steps:

[0058] a) Preparation of blueberry extract: select wild blueberry berries, wash them with running water, squeeze the juice, filter with a 200-mesh filter cloth, and make blueberry puree; add 0.03 times the quality of blueberry puree to the blueberry puree. protease, at a pH of 11 and a temperature of 40° C. for 2 hours for enzymatic hydrolysis to obtain enzymatically hydrolyzed blueberry pulp; adding an extraction solvent containing ethanol and petroleum ether to the enzymolyzed blueberry pulp, and the enzymolyzed blueberry pulp The solid-to-liquid ratio of the slurry and the extraction solvent is 1:8 (g/mL), extracted at 25°C for 12 hours, and left to stand for 5 hours, with obvious stratification; release the lower aqueous phase and collect it for later use; distill the upper petroleum ether phase to remove petroleum ether, to obtain the organic phase extract; add 0.5 times the volume of distilled water to the lower aqueous phase to continue the extraction at room temperature for 30 minutes, use a 200-mesh filter cloth to filter and remove the residue, and obtain the aqueous phase extract; extract the aqueous phase extract with the organic phase mixed liquid, add 20% of the total mass of cyclodextrin, mix evenly, spin-steam for 1.5 hours, and freeze-dry to obtain blueberry extract; the extraction solvent is composed of petroleum ether and ethanol with a volume fraction of 60%, wherein petroleum The volume ratio of ether and ethanol is 5:1;

[0059] b) Weigh blueberry extract, seabuckthorn, medlar, whole plant of dandelion, arrow mushroom, lily according to mass ratio;

[0060] c) Mix the seabuckthorn, wolfberry fruit, whole dandelion plant, arrow mushroom and lily obtained in step b), place in a sandwich pot, add water 10 times the total mass of medicinal materials, stir and mix thoroughly, and soak for 6 hours; then heat to 90 °C, decoct for 2 hours, filter with a 200-mesh filter cloth, and take the filtrate; concentrate the filtrate under reduced pressure at 60 °C and an absolute pressure of 0.03 MPa to an extract with a relative density of 1.20;

[0061] d) Mix the blueberry extract obtained by weighing in step b) with the extract prepared in step c), and freeze-dry to obtain blueberry anti-cancer brewed drink.

[0062] Example 2

[0063] The raw materials used to prepare the blueberry anti-cancer drink are: 60wt% of blueberry extract, 5wt% of seabuckthorn, 5wt% of medlar, 10wt% of dandelion whole plant, 10wt% of citrus mushroom, and 10wt% of lily.

[0064] The processing method of the blueberry anti-cancer brewed drink comprises the following steps:

[0065] a) Preparation of blueberry extract: select wild blueberry berries, wash them with running water, squeeze the juice, filter with a 200-mesh filter cloth, and make blueberry puree; add 0.03 times the quality of blueberry puree to the blueberry puree. protease, at a pH of 11 and a temperature of 40° C. for 2 hours for enzymatic hydrolysis to obtain enzymatically hydrolyzed blueberry pulp; adding an extraction solvent containing ethanol and petroleum ether to the enzymolyzed blueberry pulp, and the enzymolyzed blueberry pulp The solid-to-liquid ratio of the slurry and the extraction solvent is 1:8 (g/mL), extracted at 25°C for 12 hours, and left to stand for 5 hours, with obvious stratification; release the lower aqueous phase and collect it for later use; distill the upper petroleum ether phase to remove petroleum ether, to obtain the organic phase extract; add 0.5 times the volume of distilled water to the lower aqueous phase to continue the extraction at room temperature for 30 minutes, use a 200-mesh filter cloth to filter and remove the residue, and obtain the aqueous phase extract; extract the aqueous phase extract with the organic phase solution, add 20% of its total mass cyclodextrin, mix evenly, spin-steam for 1.5 hours, freeze-dry to obtain blueberry extract; the extraction solvent consists of petroleum ether, ethanol with a volume fraction of 60% and 0.1mol/ The hydrochloric acid composition of L, wherein the volume ratio of petroleum ether, ethanol and hydrochloric acid is 5:1:0.09;

[0066] b) Weigh blueberry extract, seabuckthorn, medlar, whole plant of dandelion, arrow mushroom, lily according to mass ratio;

[0067] c) Mix the seabuckthorn, wolfberry fruit, whole dandelion plant, arrow mushroom and lily obtained in step b), place in a sandwich pot, add water 10 times the total mass of medicinal materials, stir and mix thoroughly, and soak for 6 hours; then heat to 90 °C, decoct for 2 hours, filter with a 200-mesh filter cloth, and take the filtrate; concentrate the filtrate under reduced pressure at 60 °C and an absolute pressure of 0.03 MPa to an extract with a relative density of 1.20;

[0068] d) Mix the blueberry extract obtained by weighing in step b) with the extract prepared in step c), and freeze-dry to obtain blueberry anti-cancer brewed drink.

[0104] Example 3

[0105] The raw materials used to prepare the blueberry anti-cancer drink are: 60wt% of blueberry extract, 5wt% of seabuckthorn, 5wt% of medlar, 10wt% of dandelion whole plant, 10wt% of citrus mushroom, and 10wt% of lily.

[0106] The processing method of the blueberry anti-cancer brewed drink comprises the following steps:

[0107] a) Preparation of blueberry extract: select wild blueberry berries, wash them with running water, squeeze the juice, filter with a 200-mesh filter cloth, and make blueberry puree; add 0.03 times the quality of blueberry puree to the blueberry puree. protease, at a pH of 11 and a temperature of 40° C. for 2 hours for enzymatic hydrolysis to obtain enzymatically hydrolyzed blueberry pulp; adding an extraction solvent containing ethanol and petroleum ether to the enzymolyzed blueberry pulp, and the enzymolyzed blueberry pulp The solid-to-liquid ratio of the slurry and the extraction solvent is 1:8 (g/mL), extracted at 25°C for 12 hours, and left to stand for 5 hours, with obvious stratification; release the lower aqueous phase and collect it for later use; distill the upper petroleum ether phase to remove petroleum ether, to obtain the organic phase extract; add 0.5 times the volume of distilled water to the lower aqueous phase to continue the extraction at room temperature for 30 minutes, use a 200-mesh filter cloth to filter and remove the residue, and obtain the aqueous phase extract; extract the aqueous phase extract with the organic phase mixed liquid, add 20% cyclodextrin of its total mass and 10% adjuvant of cyclodextrin mass, mix uniformly, spin-steam for 1.5 hours, and then freeze-dry to obtain blueberry extract; the extraction solvent consists of petroleum ether, Composition of ethanol with a volume fraction of 60% and hydrochloric acid of 0.1mol/L, wherein the volume ratio of petroleum ether, ethanol and hydrochloric acid is 5:1:0.09;

[0108] b) Weigh blueberry extract, seabuckthorn, medlar, whole plant of dandelion, arrow mushroom, lily according to mass ratio;

[0109] c) Mix the seabuckthorn, wolfberry fruit, whole dandelion plant, arrow mushroom and lily obtained in step b), place in a sandwich pot, add water 10 times the total mass of medicinal materials, stir and mix thoroughly, and soak for 6 hours; then heat to 90 °C, decoct for 2 hours, filter with a 200-mesh filter cloth, and take the filtrate; concentrate the filtrate under reduced pressure at 60 °C and an absolute pressure of 0.03 MPa to an extract with a relative density of 1.20;

[0110] d) Mix the blueberry extract obtained by weighing in step b) with the extract prepared in step c), and freeze-dry to obtain blueberry anti-cancer brew.

[0111] The adjuvant is prepared by the following method: Weigh 1g of whey protein isolate and 5g of water, mix evenly, heat in a water bath to 40°C, and stir at 100 rpm for 30 minutes to obtain a whey protein isolate solution; mix 2g of oligomeric wood Sugar was dissolved in 7g of water to obtain a xylooligosaccharide solution; the xylooligosaccharide solution was added to the whey protein isolate solution, mixed evenly, and the pH was adjusted to 10 with a 5% aqueous sodium hydroxide solution by mass fraction; The final solution was heated to 90°C at 3°C/min, and was incubated at 90°C for 3 hours; after the reaction, the reaction solution was rapidly cooled to 5°C in an ice-water bath to terminate the reaction, and then freeze-dried to obtain the adjuvant agent.

[0112] When whey protein isolate is coated on the surface of active molecules, it will form a soft and elastic film, which can better block oxygen and other gases, but whey protein has more hydrophilic groups, and its barrier performance to water vapor is relatively Difference. Under high temperature conditions, xylo-oligosaccharides can undergo saccharification grafting reaction with whey protein isolate, cross-link whey protein isolate, improve the barrier properties of whey protein isolate and xylo-oligosaccharides to make adjuvant milk, play a role to antioxidant and photostabilizing effects. In addition, low-energy xylo-oligosaccharides, in addition to reducing the content of cholesterol and triglycerides in serum, also meet the health needs of the human body due to its low calorific value and special functionality that is not easily absorbed by the human body.