Beauveria bassiana tertiary medium and preparation method thereof

Abstract

The invention discloses a tertiary medium for Beauveria bassiana and a preparation method thereof. The tertiary medium for Beauveria bassiana comprises the following raw materials in parts by weight: 20-30 parts of chaff, 20 parts of wheat bran ‑30 parts, oxytetracycline 0.001‑0.01 parts and 30‑50 parts water. Can also include KH 2 PO 4 . By adding oxytetracycline to the medium formula and the water used, most microorganisms in the tap water are killed, which further ensures the low-microbial environment state of Beauveria bassiana in the long-term medium for 5-7 days, and makes Beauveria bassiana an advantage in an open environment bacterial growth. By adopting the medium provided by the invention for tertiary culture, the produced Beauveria bassiana high sporopollen is up to 230 billion / g.

Description

technical field [0001] The invention relates to the field of preparation of Beauveria bassiana, in particular to a tertiary culture medium of Beauveria bassiana and a preparation method thereof. Background technique [0002] Beauveria bassiana (Beauveria bassiana) is an ascomycete fungus, which can be industrially cultured and produced, and the conidia produced by it can be processed into microbial pesticides. Beauveria bassiana hypersporopollen is non-toxic and tasteless, and has no environmental pollution. It has persistent infectivity to pests. Once infected, pests can continuously infect and spread; it can parasitize more than 700 species of insects from 15 orders and 149 families, which is relatively safe for humans, animals and the environment. Pests are generally not easy to develop drug resistance, and can be used together with some chemical pesticides (insecticides, acaricides, fungicides). The most successful control of Beauveria bassiana in China is the agricultu...

AI Technical Summary

Problems solved by technology

Beauveria bassiana spore powder currently produced in the market has a sporulation rate of about 100 billion / g, and the yield is low, and there are many miscellaneous bacteria. The choice of medium has a lot of influence on it