Test method of benzalkonium chloride content in wet wipes
A technology of benzalkonium chloride and a test method, applied in the field of chemical detection, can solve problems such as skin damage, inability to sterilize other items, etc., and achieve the effects of small operator error, wide application range, and credible and reliable results
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[0057] 1. System suitability test
[0058] 1. Configuration of standard solution:
[0059] 1.1 Prepare a stock solution of benzalkonium chloride standard solution with a concentration of 3 mg / ml.
[0060] 1.2 Pipette 4.0ml of benzalkonium chloride standard solution stock solution, dilute to 10ml with purified water, and prepare a standard solution of about 1.2mg / ml.
[0061] 2. Chromatographic conditions:
[0062] Instrument: Waters E2695with 2998PDA
[0063] Chromatographic column: Waters CN 4.6×250mm, 5μm
[0064] Mobile phase: acetonitrile: 0.1N sodium acetate solution (adjust the pH value to 5.0 with acetic acid) = 45:55
[0065] Detection wavelength: 262nm
[0066] Flow rate: 2ml / min
[0067] Injection volume: 20μL
[0068] Chromatographic column temperature: 35°C
[0069] 3. System suitability:
[0070] Take the blank solution (purified water) and inject 2 needles. Take the standard solution, inject 6 needles, calculate the separation degree of C12 and C14, and...
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