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A Fluorescence Microscope Objective Lens with Large Field of View and High Resolution

A fluorescence microscope, high-resolution technology, applied in the optical field, can solve the problems of inability to meet the needs of life science research, mismatch of edge and center magnification, large edge distortion in the wide field of view, etc., to reduce procurement difficulty and manufacturing costs, small Relative distortion, the effect of high imaging resolution

Active Publication Date: 2021-01-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of lens off-axis aberration, the edge and center magnification do not match during the multi-lens imaging process, resulting in large edge distortion of wide field of view
At present, there are inherent constraints on the large field of view and high-resolution indicators of the objective lens, which cannot be optimized at the same time, and cannot meet the research needs of life sciences. Improvements and breakthroughs are urgently needed

Method used

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  • A Fluorescence Microscope Objective Lens with Large Field of View and High Resolution
  • A Fluorescence Microscope Objective Lens with Large Field of View and High Resolution
  • A Fluorescence Microscope Objective Lens with Large Field of View and High Resolution

Examples

Experimental program
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Embodiment 1

[0026] Embodiment 1: see Figure 1-Figure 5 , the present embodiment relates to a fluorescent microscope objective lens with a large field of view and high resolution. The magnification of the fluorescent microscope objective lens is set to 35 times, the numerical aperture is 0.5, the wavelength visible light range is 505nm-630nm, the object-side field of view diameter is 10mm, and the object-side resolution 0.4 μm. The initial structure of the fluorescence microscope objective lens is based on the structure of the microscopic objective lens while taking into account the structural characteristics of the lithography objective lens; the objective lens of the fluorescence microscope is a water immersion objective lens, which achieves a large numerical aperture and provides improved diffraction-limited spatial resolution. The water immersion objective lens It is required that the front surface of the first plano-convex lens L1 is convex or flat, so as to ensure that no air bubble...

Embodiment 2

[0034] refer to Figure 6-Figure 10 , the present embodiment relates to a fluorescent microscope objective lens with a large field of view and high resolution. The magnification of the fluorescent microscope objective lens is set to 10 times, the numerical aperture is 0.3, the wavelength visible light range is 505nm-630nm, the object-side field of view diameter is 4mm, and the object-side resolution 2 μm.

[0035] Different from Example 1, the fluorescence microscope objective lens is a dry objective lens, and only a lens or lens group is placed between the slide glass S and the dichroic mirror D. like Figure 6-Figure 7 As shown, the fluorescence microscope objective lens includes slide glass S, air A, spherical lenses L16-L28, dichroic mirror D, and emission filter E arranged in sequence from the object plane to the image plane.

[0036] Image Quality Analysis of Fluorescence Microscope Objectives Figure 8-Figure 10 . like Figure 8 As shown, the MTF value of the modul...

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Abstract

The invention discloses a large-view field high-resolution fluorescence microscope objective lens. The large-view field high-resolution fluorescence microscope objective lens comprises a slide S, a dichroscope D and an emission filter E which are arranged sequentially from an object plane to an image plane. At least one position selected from the position between the slide S and the dichroscope D,the position between the dichroscope D and the emission filter E and the position after the emission filter E is provided with a lens or a lens group. The obtained fluorescence microscope objective lens has a magnification of 10 to 35 times, a numerical aperture of 0.3 to 0.5, a wavelength visible light range of 480 nm to 680 nm, an object view field diameter of 4 mm to 10 mm and an object resolution of 0.4 mum to 2 mum. The MTF value of the fluorescence microscope objective lens is close to a diffraction limit in the full view field range of a working band, the relative distortion is less than 0.3% in the full view field range of the working band, and compared with the traditional objective lens with the same view field, the large-view field high-resolution fluorescence microscope objective lens disclosed in the invention has a higher imaging resolution and a smaller relative distortion.

Description

technical field [0001] The invention belongs to the field of optics, in particular to a fluorescent microscope objective lens with a large field of view and high resolution. Background technique [0002] To examine biological objects and tissue structures, microscope objectives with large numerical apertures are used to resolve subtle structures. However, the existing microscopes (traditional optical microscopes, electron microscopes, confocal microscopes and even the most advanced two-photon microscopes in recent years), cameras and cameras, and in vivo imaging equipment are difficult to achieve wide-field imaging at high resolution. In order to obtain wide-field imaging at high resolution, it is necessary to increase the aperture of the imaging optical system. However, due to the influence of the off-axis aberration of the lens, the edge and center magnifications do not match during the multi-lens imaging process, resulting in large edge distortion of the wide field of vi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/02
CPCG02B21/02
Inventor 沈亦兵白剑胡静王政松尾
Owner ZHEJIANG UNIV
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