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Application of ertapenem sodium in preparation of medicine for preventing and treating bovine infectious rhinotracheitis

A technology for ertapenem sodium and rhinotracheitis is applied in the application field of ertapenem sodium in the preparation of medicines for preventing and treating bovine infectious rhinotracheitis, and can solve problems such as hidden safety hazards, eradication of IBR obstacles, and inability to remove viruses.

Inactive Publication Date: 2021-08-03
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Individuals after immunization with inactivated vaccines cannot be differentially diagnosed with naturally infected individuals, so it will bring certain obstacles to the eradication of IBR
Traditional attenuated vaccines have the phenomenon of virulence returning to strength, and there are certain safety hazards
The most important thing is that bovine infectious rhinotracheitis virus is an intracellular proliferating virus, and inactivated vaccines cannot remove the virus in the body, but can only prevent

Method used

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  • Application of ertapenem sodium in preparation of medicine for preventing and treating bovine infectious rhinotracheitis
  • Application of ertapenem sodium in preparation of medicine for preventing and treating bovine infectious rhinotracheitis
  • Application of ertapenem sodium in preparation of medicine for preventing and treating bovine infectious rhinotracheitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 virus-TCID 50 Determination of

[0042] MDBK cells (preserved by Dairy Cow Research Center, Shandong Academy of Agricultural Sciences) were digested and mixed with 1 × 10 per well. 5 Cells / mL were seeded into 96-well cell culture plates, placed in 37°C, 5% CO 2 After being cultured into a single layer of cells in the cell incubator, the cell growth solution in the well was discarded, and the virus dilution solution of bovine infectious rhinotracheitis virus (preserved by the Dairy Cow Research Center, Shandong Academy of Agricultural Sciences) was serially diluted 10 times (dilutions were respectively for 10 -1 ~10 -10 ) inoculated in a 96-well plate full of monolayer cells, 100 μL per well, placed in 37°C, 5% CO 2 Continue culturing in an incubator, observe the cytopathic effect (CPE) of the cells daily, and record the number of cytopathic wells in detail. At the same time, a normal cell control group and a blank control group were set up, with 8 repli...

Embodiment 2

[0052] Example 2 Toxicity test of ertapenem sodium on MDBK cells:

[0053] MDBK cells are susceptible cells to bovine infectious rhinotracheitis virus. Therefore, the cytotoxicity of ertapenem sodium to MDBK cells was first detected, and the specific experimental steps were as follows:

[0054] (1) Inoculate 100 μL cells (MDBK 5000 cells / well) in a 96-well plate.

[0055] (2) After culturing for about 12 hours, the next step of drug addition analysis was carried out. The medium was discarded, and 100 μL of 2% FBS DMEM containing different drug concentrations (starting at 50 μM, serially diluted twice to obtain 9 concentrations of 25 μM, 12.5 μM, 6.25 μM, and 3.125 μM) was added to each well. Do 3 parallels for each concentration. At the same time, control wells: add 100 μL of 2% FBS DMEM medium containing 0.9% DMSO. Zero well: no cells are plated.

[0056] (3) At 37°C, 5% CO 2 After culturing for 48 h under the condition, the medium in the well was discarded. Wash twi...

Embodiment 3

[0061] Example 3 Inhibition experiment of ertapenem sodium on bovine infectious rhinotracheitis virus:

[0062] (1) Inoculate 1×10 in each well of a 96-well plate 4 MDBK cells, 37°C, 5% CO 2 Cultivate overnight in an incubator;

[0063] (2) Discard the medium and add 100 μL 100TCID to each well 50 Bovine infectious rhinotracheitis virus virus dilution (use 2% FBS DMEM cells to overgrow and add virus dilution, according to 50 μ M initial concentration, two-fold concentration gradient dilution adds medicine, 5% CO 2 Cultivated in an incubator;

[0064] (3) After 48 hours, operate according to the instructions of the CCK-8 kit, and measure the OD value at 450 nm with a microplate reader.

[0065] (4) analysis data, virus inhibition rate (%)=(drug treatment group D450nm value-virus control group D450nm value) / (normal cell control group D450nm value-virus control group D450nm value) * 100%, get with GraphPad Prism5 software The half effective concentration of the compound (E...

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Abstract

The invention provides the application of ertapenem sodium in the preparation of medicines for preventing and treating bovine infectious rhinotracheitis. Ertapenem sodium can inhibit and kill bovine infectious rhinotracheitis virus on the MDBK cell model in vitro, can effectively inhibit the invasion and replication of bovine infectious rhinotracheitis virus, and has low cytotoxicity, and can be used as a class of The new drug against bovine infectious rhinotracheitis virus has laid a foundation for the prevention and control of bovine infectious rhinotracheitis disease and drug research and development, and has also laid an experimental foundation for the development of highly effective and specific anti-bovine infectious rhinotracheitis virus drugs. Offer new perspectives.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of ertapenem sodium in the preparation of medicines for preventing and treating bovine infectious rhinotracheitis. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Infectious bovine rhinotracheitis (IBR), also known as necrotizing rhinitis and red nose disease, is an acute, febrile, contact infection caused by infectious bovine rhinotracheitis virus (IBRV). It is characterized by high fever, dyspnea, runny nose, inflammation of the upper respiratory tract, trachea, and mucous membranes. In the early 1950s, infectious cattle disease with symptoms of rhinotracheitis appea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/407A61P11/02A61P11/00A61P31/22
CPCA61K31/407A61P11/00A61P11/02A61P31/22
Inventor 楚会萌程凯慧杨宏军任亚初解晓莉张亮于志君孙阳阳
Owner DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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