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Hydro lifting instrument injection umbilical cord mesenchymal stem cell preparation and application thereof

A stem cell and stem cell technology, applied in animal cells, vertebrate cells, cell dissociation methods, etc., can solve problems accompanied by pain and bruises, sequelae, and treat symptoms rather than the root cause, so as to improve pathological conditions and increase blood content. The amount of water, the effect is remarkable and long-lasting effect

Inactive Publication Date: 2019-04-19
深圳市旷逸生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, drug cosmetology and plastic surgery are both temporary measures that treat the symptoms but not the root cause, with high risks, complications, pain and bruising, and possible sequelae

Method used

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  • Hydro lifting instrument injection umbilical cord mesenchymal stem cell preparation and application thereof
  • Hydro lifting instrument injection umbilical cord mesenchymal stem cell preparation and application thereof
  • Hydro lifting instrument injection umbilical cord mesenchymal stem cell preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example provides a method for establishing a safe, effective, and sufficient number of cell banks. The cells in the cell bank are certified by a professional third party, and the amount of cells can meet the continuous supply of clinical treatment.

[0042] The umbilical cord tissue is obtained after the newborn is born, and the donor must undergo a strict physical examination, including various virus tests and infectious disease tests, and sign an informed consent form and a donation agreement. In the sterile operating room, the umbilical cord freshly separated from the human body is quickly transferred to 4°C sterile sodium chloride injection, sealed, and transported to a professional cell preparation center through a cold chain within 4 hours.

[0043] In this embodiment, the specific steps for establishing the cell bank are as follows:

[0044] (1) Transfer the umbilical cord tissue to a pharmaceutical-grade GMP workshop, undergo strict treatment, sterilize wit...

Embodiment 2

[0054] This example provides a method for preparing clinical-grade umbilical cord mesenchymal stem cells using a cell factory, and the specific steps are as follows:

[0055] (1) Take 1 tube from the stem cell seed bank and pack 5-10×10 6 Cells / ml / tube of P2 generation umbilical cord mesenchymal stem cells, after recovery, press 5~10×10 3 / cm 2 The density is inoculated into a 2-layer cell factory (NEST, Cat No.771101) for stem cell expansion culture, so that the stem cells can be expanded to a certain number (P3 generation), wherein: the source of stem cells inoculated in each 2-layer cell factory For the cells in the same cell cryopreservation tube, the total number of cells inoculated in each 2-layer cell factory is 6.395×10 6 The volume of cell suspension added to each layer is 150-200ml, and the culture conditions are: at 37°C, 5% CO 2 Adhesive culture in an incubator with saturated humidity for 3 to 5 days;

[0056] (2) When the degree of cell confluence reaches abou...

Embodiment 3

[0061] This embodiment provides a method for identifying the umbilical cord mesenchymal stem cells prepared in Example 2, specifically as follows:

[0062] In 2006, the International Society for Cellular Therapy (ISCT) established the identification criteria for mesenchymal stem cells as:

[0063] (1) grow adherently in plastic petri dishes, and proliferate highly in medium containing serum;

[0064] (2) Express cell surface markers CD105, CD73, CD90, but not CD45, CD34, CD14 or CD11b, CD79a or CD19;

[0065] (3) It can differentiate into osteoblasts, adipocytes and chondrocytes in vitro.

[0066] In this example, stem cell phenotype identification is carried out according to this minimum standard.

[0067] After the proliferation and confluence of stem cells reached 90% in the 10-layer cell factory, use the multi-layer cell culture machine observation platform to observe the cell morphology, such as figure 1 As shown, the stem cells grow adherently, spindle-shaped, and are n...

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Abstract

The invention discloses a hydro lifting instrument injection umbilical cord mesenchymal stem cell preparation and application thereof. The umbilical cord mesenchymal stem cell preparation is a stem cell suspension composed of umbilical cord mesenchymal stem cells and normal saline, wherein the total volume of the umbilical cord mesenchymal stem cells and the normal saline is 5 ml, and the total number of umbilical cord mesenchymal stem cells in the dry cell suspension is 1*10<6>-10<7>. A 9-needle type hydro lifting injection instruction is adopted, on the basis of the vacuum negative pressuretechnology, average coverage is performed on each unit area (1 cm<2>) through the same dosage, it is a new breakthrough that traditional point filling is replaced with face filling, umbilical cord mesenchymal stem cells are injected into a skin corium layer through the hydro lifting injection instrument, fixed-point, quantified and fixed-layer injection is performed, and the preparation has the effects of being free of traces and pain, uniform in injection, and convenient and rapid to use, performing treatment only for 10 min, and achieving the skin beautifying effects of smoothing skin, removing wrinkels, resisting skin aging and the like.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and relates to a hydrolight injector for injecting umbilical cord mesenchymal stem cell preparation and its application in skin rejuvenation treatment. Background technique [0002] The aging of the skin and the aging of the body are carried out simultaneously. At present, the mechanism of skin aging is relatively representative, including the theory of gene regulation of skin aging, the theory of free radicals, the theory of metabolic disorders, and the effect of photoaging. Although the exact mechanism of skin aging is unclear, more and more researches believe that active oxygen is a key factor leading to aging. Based on the traditional theory of free radical aging, Sohal et al. put forward the "oxidative stress (oxidative stress) aging theory", thinking that excessive active oxygen can destroy the balance between the production and removal of active oxygen in tissues, and can destroy the str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P17/18A61P17/00C12N5/0775
CPCA61K9/0021A61K35/28A61P17/00A61P17/18C12N5/0665C12N2509/00
Inventor 王冰张伟涛王康沈洁沈艳
Owner 深圳市旷逸生物科技有限公司
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