Method for differentiating placenta wall decidual mesenchymal stem cells into endometrial epithelial cells

A technology of mesenchymal stem cells and endometrium, applied in the field of stem cells and regenerative medicine, can solve the problems of difficult large-scale cultivation, difficult to meet seed cells, and low proliferation activity of endometrial epithelial cells

Inactive Publication Date: 2019-04-19
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the existing endometrial epithelial cells are difficult to obtain. Most of the isolated endometrial epithelial cells collect tissue samples through dilatation and curettage, which will cause great damage to patients.
In addition

Method used

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  • Method for differentiating placenta wall decidual mesenchymal stem cells into endometrial epithelial cells
  • Method for differentiating placenta wall decidual mesenchymal stem cells into endometrial epithelial cells
  • Method for differentiating placenta wall decidual mesenchymal stem cells into endometrial epithelial cells

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Embodiment Construction

[0028] The technical solutions of the present invention will be further detailed below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments.

[0029] It is worth noting that the method for differentiating amniotic fluid mesenchymal stem cells into neural stem cells provided by the present invention is not used for disease diagnosis and treatment purposes.

[0030] Unless otherwise specified, the reagents used in the present invention are all existing reagents and can be obtained through commercial channels. For the purpose of brevity, some technical operations are not specifically described in detail, but it should be understood that these operations are within the scope well known to those skilled in the art, which can be implemented according to the content recorded in this specification.

[0031] Differentiation of amniotic fluid mesenchymal stem cells into neural stem cells is as follows:

[0032] 1. The hospital collects ...

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Abstract

The invention provides a method for differentiating placental wall decidual mesenchymal stem cells into endometrial epithelial cells, which comprises the following steps of: adding a tissue digestivejuice into placental wall decidual tissues for digestion, filtering to remove undigested tissue blocks after the digestion is terminated, collecting cell suspension, centrifuging, removing the supernatant, cleaning the precipitate and centrifuging again to obtain mesenchymal stem cells of placenta wall fetal membrane tissues; culturing the placental wall decidual mesenchymal stem cells by using aselective culture medium of the placental wall decidual mesenchymal stem cells; passing the primary cells of the placental wall decidual mesenchymal stem cells to P3 generation; taking the P3 generation placental wall decidual mesenchymal stem cells, adding the placental wall decidual mesenchymal stem cells into a selective culture medium, and inoculating and culturing overnight; removing the selective culture medium of the placental wall decidual mesenchymal stem cells, and carrying out induction culture by using the endometrial epithelial cell differentiation culture medium to obtain the endometrial epithelial cells. The method can successfully obtain endometrial epithelial cells with cell activity.

Description

Technical field [0001] The invention belongs to the field of stem cells and regenerative medicine, and specifically relates to a method for differentiating placental wall decidual mesenchymal stem cells into endometrial epithelial cells. Background technique [0002] The female uterus is an organ with high regenerative capacity. During the reproductive age of an adult female, it has to undergo more than 400 proliferation, differentiation and shedding to form menstruation. The endometrium is composed of epithelial cells, mesenchymal cells, and blood vessels. Endometrial epithelial cells are an important part of endometrial tissue. With the occurrence of menstrual period, they are an important part of maintaining uterine function. The proliferation and differentiation of endometrial epithelial cells play an important role in the field of endometrial injury repair and tissue engineering. [0003] Under normal circumstances, the new cells required for the periodic proliferation and sh...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2500/30C12N2501/11C12N2501/115C12N2501/12C12N2501/15C12N2506/025C12N2509/00
Inventor 朱建斌王刚江嘉豪何美弟陈炽峰康斯敏陈碧莹李静静蔡祥胜王进辉
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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