Method for differentiating placenta wall decidual mesenchymal stem cells into endometrial epithelial cells

Abstract

The invention provides a method for differentiating placental wall decidual mesenchymal stem cells into endometrial epithelial cells, which comprises the following steps of: adding a tissue digestivejuice into placental wall decidual tissues for digestion, filtering to remove undigested tissue blocks after the digestion is terminated, collecting cell suspension, centrifuging, removing the supernatant, cleaning the precipitate and centrifuging again to obtain mesenchymal stem cells of placenta wall fetal membrane tissues; culturing the placental wall decidual mesenchymal stem cells by using aselective culture medium of the placental wall decidual mesenchymal stem cells; passing the primary cells of the placental wall decidual mesenchymal stem cells to P3 generation; taking the P3 generation placental wall decidual mesenchymal stem cells, adding the placental wall decidual mesenchymal stem cells into a selective culture medium, and inoculating and culturing overnight; removing the selective culture medium of the placental wall decidual mesenchymal stem cells, and carrying out induction culture by using the endometrial epithelial cell differentiation culture medium to obtain the endometrial epithelial cells. The method can successfully obtain endometrial epithelial cells with cell activity.

Description

Technical field [0001] The invention belongs to the field of stem cells and regenerative medicine, and specifically relates to a method for differentiating placental wall decidual mesenchymal stem cells into endometrial epithelial cells. Background technique [0002] The female uterus is an organ with high regenerative capacity. During the reproductive age of an adult female, it has to undergo more than 400 proliferation, differentiation and shedding to form menstruation. The endometrium is composed of epithelial cells, mesenchymal cells, and blood vessels. Endometrial epithelial cells are an important part of endometrial tissue. With the occurrence of menstrual period, they are an important part of maintaining uterine function. The proliferation and differentiation of endometrial epithelial cells play an important role in the field of endometrial injury repair and tissue engineering. [0003] Under normal circumstances, the new cells required for the periodic proliferation and sh...

AI Technical Summary

Problems solved by technology

However, the existing endometrial epithelial cells are difficult to obtain. Most of the isolated endometrial epithelial cells collect tissue samples through dilatation and curettage, which will cause great damage to patients.

In addition, the proliferative activity of endometrial epithelial cells is low, and it is difficult to cultivate them on a large scale, and it is difficult to meet the seed cells required for later tissue engineering to repair damaged endometrium.

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