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Maltose functionalized nanocomposite material based on ion-complementary peptide self-assembly and its preparation method and application

A nanocomposite material and nanomaterial technology, applied in the field of maltose functionalized nanocomposite materials, can solve problems such as harsh conditions, cumbersome processing steps, and strong surface dependence, and achieve mild conditions, environmentally friendly process, and simple preparation methods Effect

Active Publication Date: 2022-02-25
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, almost all functional groups reported are stepwise chemically bonded to the surface of nanomaterials, which requires cumbersome processing steps, strong surface dependence, and harsh conditions (non-aqueous solvents, high temperatures, etc.)

Method used

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  • Maltose functionalized nanocomposite material based on ion-complementary peptide self-assembly and its preparation method and application
  • Maltose functionalized nanocomposite material based on ion-complementary peptide self-assembly and its preparation method and application
  • Maltose functionalized nanocomposite material based on ion-complementary peptide self-assembly and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Synthesis of maltose-modified ion-complementary peptides

[0040] (1) Add 0.174g Fmoc-Rink Amide MBHA Resin to the solid phase synthesis tube, add 5mL of dichloromethane to soak the resin for 30 minutes, fully swell, remove the dichloromethane by suction filtration, add 5mL of piperidine and N to the solid phase synthesis tube , the volume ratio of N-dimethylformamide is a mixed solution of 1:4, reacted for 20 minutes, the mixed solution was removed by suction filtration, and the volume ratio of adding 5mL piperidine and N,N-dimethylformamide was 1: The mixture of 4 was reacted for 20 minutes, filtered with suction, and the resin was washed twice with dichloromethane and 3 times with N,N-dimethylformamide, each 5 mL, to complete the removal of Fmoc-Rink Amide MBHA Resin from Fmoc-Rink Amide MBHA Resin. time, and then add 5mL N,N-dimethylformamide, 0.1331g Fmoc-Lys(Boc)-OH, 0.03837g 1-hydroxybenzotriazole, 0.1077g benzotriazole-N,N,N′ ,N'-tetramethylurea tetrafluorob...

Embodiment 2

[0053] In this example, C@Fe is used 3 O 4 Nanoparticles (prepared according to the method disclosed in the literature "Yang, J.; Li, J.; Qiao, J.; Lian, H.; Chen, H.J. Chromatogr. A 2014, 1325, 8-15") were replaced and implemented SiO in Example 1 2 @Fe 3 O 4 Nanoparticles, other steps are the same as in Example 1 to obtain maltose-functionalized C@Fe 3 O 4 composite material.

[0054] The Fe in the above-mentioned Examples 1 and 2 was 3 O 4 , SiO 2 @Fe 3 O 4 , C@Fe 3 O 4 , Maltose functionalized SiO 2 @Fe 3 O 4 Composites and Maltose-functionalized C@Fe 3 O 4 The composite materials were subjected to Fourier transform attenuated total reflection infrared spectroscopy and X-ray photoelectron spectroscopy, and the results were as follows Figure 2 to Figure 6 shown. It can be seen from the figure that the maltose functionalized SiO 2 @Fe 3 O 4 Composites and Maltose-functionalized C@Fe 3 O 4 Composite (580cm -1 At Fe-O, 1100cm -1 Si-O-Si at 1740 and 1...

Embodiment 3

[0056] Application of Maltose-functionalized Nanocomposites Obtained in Examples 1 and 2 in Glycopeptide Enrichment

[0057] 1. Comparative experiment of enrichment performance of different materials

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Abstract

The invention discloses a maltose functional nanometer material based on ion-complementary peptide self-assembly, a preparation method and an application thereof. In the present invention, the ion-complementary peptide with alternate arrangement of hydrophilic and hydrophobic residues modified on maltose is placed on SiO 2 @Fe 3 o 4 and C@Fe 3 o 4 Self-assembly on the surface of other nanomaterials to form stable maltose-functionalized nanocomposites. The composite material of the invention can selectively enrich standard glycoproteins and glycopeptides in enzymatic hydrolysis solutions of biological samples, has high enrichment efficiency, good selectivity, low detection limit, stable performance and can be reused. The preparation method of the composite material is simple, the conditions are mild, green and non-toxic, and can be used for one-step functionalization of the surface of various nano-enriched materials.

Description

technical field [0001] The invention belongs to the technical field of surface functionalization of nanomaterials, and in particular relates to a maltose functionalized nanocomposite material based on self-assembly of ionic complementary peptides, a preparation method of the material, and an application of using the material to selectively enrich glycopeptides. Background technique [0002] Protein glycosylation is one of the most prevalent and complex post-translational modifications involved in many biological processes, contains a wealth of information related to mammalian and genetic diseases, and is a hallmark of cancer and other diseases. Therefore, comprehensive analysis of glycoproteins / glycopeptides is required for biological function and disease studies. At present, mass spectrometry has become the most effective tool to provide sugar structures, glycosylation sites and corresponding sugar chain heterogenes in glycoproteomics research. However, the native abundanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08C07K9/00
CPCC07K7/06C07K7/08C07K9/00
Inventor 党福全张莉李南石海兰
Owner SHAANXI NORMAL UNIV
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