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Portable detection kit for alkaline phosphatase based on temperature change and its application

A portable detection and detection reagent technology, applied in the field of biosensing, which can solve the problems of low sensitivity and complex surface modification

Active Publication Date: 2020-06-16
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the colorimetric method is simple to operate, the detection sensitivity is low. Although the sensitivity of other methods has been improved, there are still some shortcomings, such as the need for large instruments, complex surface modification and labeling, etc.

Method used

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  • Portable detection kit for alkaline phosphatase based on temperature change and its application
  • Portable detection kit for alkaline phosphatase based on temperature change and its application
  • Portable detection kit for alkaline phosphatase based on temperature change and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A portable alkaline phosphatase detection kit based on temperature changes of the present invention, comprising: 20 μg / mL manganese dioxide nanosheets, 23 μg / mL L-ascorbic acid-2-phosphate trisodium salt, 0.48 mg / mL Magnesium chloride and 20 μg / mL dopamine.

[0047] An application of the alkaline phosphatase portable detection kit of the present embodiment in the detection of alkaline phosphatase, its application method comprises the following steps:

[0048] (1) Add manganese dioxide nanosheets and L-ascorbic acid-2-phosphate trisodium salt into Tris-HCl buffer solution ((the composition of Tris-HCl buffer solution is: 10mmol / L Tris-HCl buffer, pH 7.4) A detection reagent was obtained, the concentration of manganese dioxide nanosheets in the detection reagent was 20 μg / mL, and the concentration of L-ascorbic acid-2-phosphate trisodium salt was 23 μg / mL.

[0049] (2) Prepare a standard solution of alkaline phosphatase: use Tris-HCl buffer as a solvent, and prepare a co...

Embodiment 2

[0060] Investigate the influence of different buffer solutions on the detection effect:

[0061] Manganese dioxide nanosheets and L-ascorbic acid-2-phosphate trisodium salt were added to the phosphate buffer (the composition of the phosphate buffer is: 10mmol / L phosphate buffer, pH 7.4, the composition of the phosphate buffer is the same throughout the text) , Tris-HCl buffer (the composition of Tris-HCl buffer is: 10mmol / L Tris-HCl buffer, pH 7.4, the composition of the phosphate buffer is the same throughout the text), HEPES buffer (10mmol / L HEPES buffer, pH 7.4 , the composition of the HEPES buffer solution is the same throughout the text), and detected according to the method of Example 1.

[0062] Test results such as Figure 4 Shown: The temperature change value is the largest and the signal is the strongest in Tris-HCl buffer. Therefore, Tris-HCl buffer is selected as the best buffer in subsequent examples.

Embodiment 3

[0064] Investigate the influence of dopamine solution concentration on the detection effect:

[0065] Dopamine was dissolved in Tris-HCl buffer, and different concentrations of dopamine solutions were prepared: 0 μg / mL, 5 μg / mL, 10 μg / mL, 15 μg / mL, 20 μg / mL, 30 μg / mL and 40 μg / mL. According to the steps of Example 1, different concentrations of dopamine solutions were added for detection.

[0066] Test results such as Figure 5 As shown: when the dopamine concentration in the detection reagent is 20-40 μg / mL, it reaches a plateau, the temperature change value is the largest, and the signal is the strongest. Therefore, 20 μg / mL is selected as the optimal concentration of dopamine solution in subsequent examples.

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Abstract

The invention discloses a portable alkaline phosphatase detection kit based on temperature change and application thereof. The portable alkaline phosphatase detection kit comprises manganese dioxide nanoflakes, L-ascorbic acid-2-trisodium phosphate salt, dopamine and a buffer solution. The detection kit utilizes a portable near-infrared laser to irradiate reaction products; based on the temperature change of the reaction products, sensitive detection of a target can be realized through a low-cost thermometer or discoloration temperature measuring patches; and the portable alkaline phosphatasedetection kit can be used for detecting alkaline phosphatase, and has the advantages of simplicity and convenience, quickness, good selectivity, high sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of biosensing, in particular to a portable alkaline phosphatase detection kit based on temperature change and an application thereof. Background technique [0002] In recent years, research on portable sensing (without large instruments and professional operators) for rapid and low-cost detection of targets (such as proteins, nucleic acids, small molecules, and ions) at the site of the incident or at home and in remote areas has become a research topic. hotspot. The research and development of such products is of great significance to the fields of scientific research, clinical testing and biosensing. With the rapid development of science and technology, point-of-care testing (POCT) has become popular all over the world, and many products have been commercialized. However, there are only a few relatively mature portable sensors at present, which cannot meet people's needs. Therefore, it is very urgent to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/359G01N21/3577
CPCG01N21/3577G01N21/359G01N21/78
Inventor 王青刘晓峰王柯敏羊小海邹利媛
Owner HUNAN UNIV
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