Promotor of plutella xylostella Bt (Bacillus Thuringiensis) insecticidal protein Cry1Ac resistant gene MAP4K4 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4) and application thereof

Abstract

The invention discloses a core promotor sequence for regulating expression of a plutella xylostella Bt (Bacillus Thuringiensis) Cry1Ac insecticidal protein resistant gene MAP4K4 (Mitogen-activated Protein Kinase Kinase Kinase Kinase 4), mutation sites and application of the core promotor sequence in plutella xylostella Bt Cry1Ac insecticidal protein resistance identification and field monitoring.The mutation sites are nucleotides located at a 491 site, a 474 site and 469 to 470 sites at the upstream of an initial codon ATG and are respectively named M1, M2 and M3. A nucleotide sequence of plutella xylostella Bt Cry1Ac insecticidal protein susceptible population promotor is as shown in SEQ ID NO.1, and a nucleotide sequence of a resistance near-isogenic line population promotor is as shownin SEQ ID NO.2. An experiment verifies that the activity of a MAP4K4 gene promotor is remarkably enhanced when M2 and M3 are in mutation at the same time. According to the core promotor sequence disclosed by the invention, the research of promotor mutation in the field of insect Bt resistance is promoted, and particularly, important theoretical and practical significances in aspects of insect Btresistance field detection and control are obtained.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the core promoter of the key gene MAP4K4 of diamondback moth Bt resistance and its application. Background technique [0002] Diamondback moth Plutella xylostella (L.), belonging to Lepidoptera (Lepidoptera) family Plutellidae, is an important agricultural pest in the world, mainly damaging cruciferous vegetables and crops. Plutella xylostella has a small body, short generation cycle, and strong reproductive ability. It mainly drills into the mesophyll to feed on vegetables in the larval stage, forming cavities and nicks, affecting the quality and yield of vegetables. When the damage is serious, it can cause more than 90% reduction in vegetable production, or even crop failure. At present, the annual cost of controlling diamondback moth worldwide is as high as 4-5 billion US dollars. The reason why the diamondback moth can cause such serious damage is mainly because it has pro...

AI Technical Summary

Problems solved by technology

But at present, there is no report on the core promoter of the MAP4K4 gene in Plutella xylostella and the identification of the mutation site of the promoter in the Bt-resistant Plutella xylostella population, but this is helpful for revealing the molecular regulation mechanism of the transcriptional overexpression of the Bt-resistant Plutella xylostella MAP4K4 gene And the field monitoring and management of Plutella xylostella Bt resistance has important theoretical and practical significance

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