Electrochemical luminescence detection dopamine method based on boron nitrogen compound
A technology for luminescence detection and dopamine, which is applied in chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc., to achieve stable reagents, simple operation, and good selectivity
Inactive Publication Date: 2019-05-03
TIANJIN UNIVERSITY OF TECHNOLOGY
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[0005] There is no precedent for the application of boron nitrogen compounds to the content detection of dopamine in the prior art
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[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that: the following examples are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.
[0029] The hydrochloric acid (HCL) dopamine powder used in the examples was purchased from Tianjin Jiangtian Uniform Technology Co., Ltd., and the magnetic stirrer and MPI-EII electrochemiluminescence analysis system multifunctional chemiluminescence detector used were purchased from Xi’an Ruimai Company in China. ; The boron nitrogen compound was prepared according to the method described in Liu Xuguang's patent number "CN201811165383.2" and the patent name "A simple and efficient method for synthesizing borazaromatic hydrocarbons".
[0030] A method for detecting dopamine by electrochemiluminescence based on boron nitrogen compounds, the specific steps are; ...
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The invention discloses an electrochemical luminescence detection dopamine method based on a boron nitrogen compound. The method comprises the following steps of uniformly mixing a sample to be testedwhich contains dopamine in a phosphate buffer solution; and uniformly laying a boron nitrogen compound powder and fixing on one side of carbon paper, uniformly laying polyaniline on one side of the reverse side of the carbon paper and drying and then taking as a working electrode, using a three-electrode system to carry out electrochemical luminescence intensity detection, and comparing the measured electrochemical luminescence intensity with a linear regression equation so as to acquire a dopamine concentration in the solution and then acquire a dopamine content in the sample to be tested. By using the electrochemical luminescence detection dopamine method based on the boron nitrogen compound, a detection limit is low, a linear range is wide, sensitivity is high and an adopted apparatusis simple. The cost of detecting the dopamine is low, the sensitivity is high, operation is simple and selectivity is good.
Description
technical field [0001] The invention relates to the field of electrochemiluminescence detection. In particular, it relates to a method for detecting dopamine by electrochemiluminescence based on boron nitrogen compounds. Background technique [0002] In the field of luminescence, the research of organic materials has been paid more and more attention by people. Organic compounds have the advantages of various types, good tunability, rich colors, high color purity, and relatively flexible molecular design, so they have good application prospects in luminescent materials. Nowadays, boron-azide chemistry has gradually become an important branch of organic semiconductor light-emitting materials. The introduction of sulfur, nitrogen and oxygen into the conjugated molecular skeleton can endow the conjugated molecules with different photoelectric properties, especially the use of carbon-nitrogen double bonds to replace carbon. Carbon double bonds give rise to different analogs of...
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IPC IPC(8): G01N27/26G01N21/76
Inventor 杨正春杨鑫刘旭光张进云廖振宇贺婕李明辰牛慧敏祁洋洋潘鹏刘君魏军
Owner TIANJIN UNIVERSITY OF TECHNOLOGY