Detection method for traditional Chinese medicine composition, and applications thereof

A detection method and composition technology, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of detection and monitoring of traditional Chinese medicine compositions, many flavors of traditional Chinese medicines, and inability to interfere with each other, and achieve effective quality performance, clear quality detection, and realization of quality effect

Active Publication Date: 2019-05-03
BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to provide a detection method and application of a traditional Chinese medicine composition, which overcomes the inability to comprehensively and clearly detect the quality of the traditional Chin

Method used

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  • Detection method for traditional Chinese medicine composition, and applications thereof
  • Detection method for traditional Chinese medicine composition, and applications thereof
  • Detection method for traditional Chinese medicine composition, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0064] Experimental Example 1 Ginsenoside Re and Ginsenoside Rg 1 Investigation of Gradient Elution Procedure in Content Determination

[0065] According to the composition and preparation method of Example 12, the granules obtained were divided into two groups in parallel, namely the test group and the control group. 1 content determination.

[0066] (1) Test group: get above-mentioned granule according to " preparation of need testing solution " method and " preparation of negative reference substance solution " method in embodiment 14, make need testing solution and negative reference substance solution, then according to implementation The disclosed "chromatographic condition" and "measurement method" of example 14 carry out the spectrum determination, and the results are as follows: figure 1 shown.

[0067] (2) Control group: get above-mentioned granule according to " the preparation of need testing solution " method and " the preparation of negative reference substanc...

experiment example 2

[0070] Experimental Example 2 Ginsenosides Re and Rg 1 Investigation on the preparation method of the test solution in the content determination

[0071]The granules obtained according to the composition of Example 12 and the preparation method are divided into three groups in parallel, respectively, the test group and the control group 1 and the control group 2, and each group has six parts, and the test group, the control group and the control group 2 are respectively as follows Methods Ginsenoside Re and Ginsenoside Rg 1 content determination.

[0072] (1) Test group: get above-mentioned granule according to " preparation of need testing solution " method and " preparation of negative reference substance solution " method in embodiment 14, make need testing solution and negative reference substance solution, then according to implementation The "chromatographic conditions" and "measurement methods" disclosed in Example 14 were used for spectrum determination.

[0073] (2...

experiment example 3

[0080] Experimental example 3 Investigation of the gradient elution procedure in the content determination of β-asarone and the identification of ligustilide

[0081] Due to the complexity of the ingredients in the traditional Chinese medicine composition of the present invention, most of which are flavonoids, saponins and polysaccharides, it is difficult to measure β-asarone and ligustilide. The effect of the desorption degree on the chromatographic analysis results.

[0082] According to the composition and preparation method of Example 12, the granules obtained were divided into two groups in parallel, namely the test group and the control group. 1 content determination.

[0083] (1) Test group: get above-mentioned granule according to " preparation of need testing solution " method and " preparation of negative reference substance solution " method in embodiment 17, make need testing solution and negative reference substance solution, then according to implementation The...

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Abstract

The invention provides a detection method for a traditional Chinese medicine composition. The traditional Chinese medicine composition includes, by weight, 4-9 parts of ginseng, 4-9 parts of fried spine date seed, 4-9 parts of honey-fried herba epimedii, 5-12 parts of prepared rehmannia root, 3-8 parts of largehead atractylodes rhizome stir-fried with bran, 3-8 parts of processed radix polygalae,4-9 parts of grassleaf sweetflag rhizome, 5-12 parts of Chinese angelica, and 1-5 parts of honey-fried liquorice root. The detection method includes taking octadecylsilane bonded silica gel as a filling agent, taking pure water as a mobile phase A, taking acetonitrile as a mobile phase B, and performing gradient elution, a detection wavelength being 198-208 nm; determining ginsenoside Rg1 and ginsenoside Re content; utilizing an HPLC method to determine effective component beta-asarone icariin content in the composition, and identifying the Chinese angelica; and utilizing a TLC method to realize qualitative identification on the prepared rehmannia root and the honey-fried liquorice root in a prescription. The method is high in accuracy and sensitivity and wide in detection scope, so that the effective controlling of drug quality can be realized; and the method can be taken as the quality monitoring method for traditional Chinese medicine compositions and preparations.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine preparations, in particular to a detection method and application of a traditional Chinese medicine composition. Background technique [0002] Alzheimer's disease (Alzheimerdisease, AD), also known as senile dementia, is a progressive and irreversible neurodegenerative disease. The disease is mainly clinically manifested by memory impairment, mental decline, executive dysfunction, and personality and behavior changes, and the onset is slow or insidious, and the incidence rate is high. It is more common in the elderly over 70 years old. The prevalence rate is 5% in the 65-year-old population, and as high as 40% in the 80-year-old population. The etiology of Alzheimer's disease is complex, which is generally the result of multiple factors such as genetic factors, environmental factors and living habits. [0003] The main clinical symptoms of dementia are progressive memory decline and c...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
Inventor 李萍肖婷婷杨华张蓓高雯候譞
Owner BEIJING ZHONGYAN TONGRENTANG CHINESE MEDICINE R & D
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