Artificial feed used for bioassay of young larvae of red palm weevils

An artificial feed and bioassay technology, which is applied in the field of bioassay feed for young larvae of the red palm weevil, can solve the problems of high cost, loss of activity of active substances when heated, uniform mixing of insecticidal proteins, etc., and achieves the advantages of convenient material collection and low cost Effect

Pending Publication Date: 2019-05-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of the feed is complex, the cost is high, and because the insecticidal protein is inactivated at a temperature exceeding 40°C, and the freezing point of ordinary agarose used in most experiments is above 40°C, the prepared artificial feed cannot be compared with insecticidal The protein is mixed evenly, and it is prone to the problem that the active substance loses its activity when heated during the preparation process, which does not meet the experimental requirements and is not applicable

Method used

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  • Artificial feed used for bioassay of young larvae of red palm weevils
  • Artificial feed used for bioassay of young larvae of red palm weevils

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Add 5 g of bagasse and 2.5 g of high-purity agarose (UltraPure agarose from Life Technologies) into a beaker containing 95 ml of sugarcane juice, heat in a microwave oven until the agarose is completely melted. Cool slightly to 50°C, add 0.15g potassium sorbate, 0.15g sodium propyl p-hydroxybenzoate, 0.27g aureomycin, 0.10g vitamin C, 0.08g glucose, 0.08g amino acid dissolved in alcohol, stir evenly, that is An artificial diet was obtained for the bioassay of young larvae of the red palm weevil.

Embodiment 2

[0031] Add 10g bagasse and 1.8g agarose high-purity agarose (UltraPure agarose from Life Technologies) into a beaker containing 90ml sugarcane juice, heat in a microwave oven until the agarose is completely melted. Cool slightly to 50°C, add 0.20g potassium sorbate, 0.15g sodium propyl p-hydroxybenzoate, 0.27g aureomycin, 0.15g vitamin C, 0.10g glucose, 0.10g amino acid dissolved in alcohol, stir evenly, that is An artificial diet was obtained for the bioassay of young larvae of the red palm weevil.

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Abstract

The invention discloses an artificial feed for young larvae of red palm weevils. The artificial feed is prepared from the following raw materials: a cellulose component, a vitamin component, a preservative component, an antibiotic component, a carbon source component, a nitrogen source component, a solvent component and a coagulant component. The artificial feed has the advantages of simple components, convenient material acquisition, simple and convenient operation and stable effect, can ensure the smooth progress of the bioassay experiment of the young larvae of red palm weevils, and has important significance for preventing and treating the invasion pest of red palm weevils.

Description

technical field [0001] The invention belongs to the field of pest biological control, and in particular relates to a bioassay feed for young larvae of the red palm weevil. Background technique [0002] The red palm weevil, belonging to the family Coleoptera, is also known as the palm weevil, rusty palm weevil, coconut beetle, coconut beetle, etc. It is considered to be a high-risk quarantine pest from outside, and it is widely endangering Guangdong, Guangxi and Hainan. , Yunnan, Fujian and other areas of palm plants. In 2005, the State Forestry Administration included the red palm weevil in the newly released list of forestry quarantine pests and prohibited its entry. [0003] Red palm weevil adults invade the tree body from the heart leaves, injured parts or lateral roots of the plant, feed on the xylem of the plant and lay eggs; the hatched larvae eat the inside of the stem and the growth point, and eat its soft tissue, resulting in damage to the damaged tissue. Necrotic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/90A23K10/30A23K10/37A23K20/163A23K20/105A23K20/195A23K20/174A23K20/168A23K20/142A23K20/10A23L3/3526
CPCY02P60/87
Inventor 吴松青周娴张飞萍夏枫胡霞王荣梁光红黄世国
Owner FUJIAN AGRI & FORESTRY UNIV
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