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Application of sodium bicarbonate buffer system in nucleic acid extraction

A sodium bicarbonate and buffer system technology, applied in the field of biomedicine, can solve the problems of reduced analytical sensitivity of nucleic acid detection kits, achieve significant technological progress, improve analytical sensitivity, and increase extraction efficiency

Pending Publication Date: 2019-05-07
SHANGHAI KEHUA BIO ENG
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  • Abstract
  • Description
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Problems solved by technology

[0005] For the above technical problems in the prior art, the invention provides the application of sodium bicarbonate buffer system in nucleic acid extraction, the application of this sodium bicarbonate buffer system in nucleic acid extraction will solve the temperature change in the prior art A technical problem that will lead to a decrease in the analytical sensitivity of the nucleic acid detection kit using the Tris-HCl buffer system as the extraction buffer

Method used

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  • Application of sodium bicarbonate buffer system in nucleic acid extraction
  • Application of sodium bicarbonate buffer system in nucleic acid extraction
  • Application of sodium bicarbonate buffer system in nucleic acid extraction

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Embodiment 1

[0018] 1. Research overview:

[0019] The main object of this study is the pH of the sodium bicarbonate buffer, and the study is divided into two parts: the determination and verification of the pH of the buffer system. In the part of determining the pH of the buffer system, a gradient search method is used to find a suitable pH in the range of pH 6.4-8.0. In the verification part of the pH of the buffer system, the thermal stability of the appropriate pH is verified to initially confirm the applicability of the pH.

[0020] The main method of this study is the functional test (component replacement method), that is, the relevant components in this product are replaced with the components to be studied, and the components to be studied are evaluated with the analytical sensitivity as the index. in,

[0021] 1.1 Analytical Sensitivity Sample Concentration:

[0022] HBV: 5IU / mL; HCV: 50IU / mL; HIV: 50IU / mL

[0023] 1.2 Sample size: 20

[0024] 1.3 Evaluation criteria: detect...

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Abstract

The present invention provides an elution buffer solution used for a nucleic acid detection kit. The elution buffer solution is a sodium hydrogencarbonate solution having a concentration of 5 mM and apH of 6.7-7.2. The present invention also provides an application of a sodium bicarbonate buffer system in nucleic acid extraction, the sodium bicarbonate buffer system is a sodium bicarbonate buffersolution having a concentration of 5 mM and a pH of 7.0. The present invention also provides an application of an arginine-containing lysate in a nucleic acid detection kit, and a concentration of arginine in the lysate is 15 mM. The provided buffer system increases analytical sensitivity of nucleic acids (primarily DNA), increases extraction efficiency of DNA without changing extraction efficiency of RNA, and thus increases the analytical sensitivity of DNA detection of the product.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a nucleic acid extraction technology, in particular to the application of a sodium bicarbonate buffer system in nucleic acid extraction. Background technique [0002] Viral hepatitis B is a hepatitis disease caused by hepatitis B virus (hereinafter referred to as HBV) infection. It is contagious and can develop into liver cancer through liver cirrhosis. It is extremely harmful to health, and effective diagnosis can prevent a large number of hepatitis B virus infections. . my country is a big country with hepatitis B, and there are currently about 120 million hepatitis B virus carriers. Therefore, the diagnosis of hepatitis B is very important. In addition, hepatitis C virus (hereinafter referred to as HCV) and human immunodeficiency virus (hereinafter referred to as HIV) also pose a huge threat to health. At present, the main detection methods for the above-mentioned viruses are immunoa...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
Inventor 丁硕方琴张健蒋淼
Owner SHANGHAI KEHUA BIO ENG
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