Method for improving detection limit of Staphylococcus aureus in easily foamable liquid food

A staphylococcus, liquid food technology, applied in the field of detection, can solve the problems of foaming, increase the sampling volume, etc., to achieve the effect of avoiding missed detection, increasing the sampling volume, and increasing the detection rate

Inactive Publication Date: 2019-05-10
HENAN BUSINESS SCI RES INST
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for improving the detection limit of Staphylococcus aureus in easily foaming liquid food, increase the sampling amount, use an effective defoamer to solve the problem of foaming in the sample pretreatment enrichment process, adopt Vacuum membrane filtration enriches the microorganisms in the sample to the microporous membrane to improve the detection limit and sensitivity of Staphylococcus aureus in food

Method used

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  • Method for improving detection limit of Staphylococcus aureus in easily foamable liquid food
  • Method for improving detection limit of Staphylococcus aureus in easily foamable liquid food
  • Method for improving detection limit of Staphylococcus aureus in easily foamable liquid food

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Embodiment 1

[0027] A method for improving the detection limit of Staphylococcus aureus in easy-to-foam liquid food, comprising the following steps:

[0028] 1) Take 500mL of liquid food samples that are prone to foaming and add 10mL of polydimethylsiloxane (PMX) to defoam, centrifuge at 8000rmp / min for 6min under sterile conditions at 20°C, and collect the supernatant;

[0029] 2) The obtained supernatant is suction-filtered with a Φ50mm 0.45μm polyether PES microporous membrane, and the microorganisms are enriched on the microporous membrane;

[0030] 3) Put the microporous filter membrane in a Φ55mm 50mL flat-bottomed glass with a stopper, wash and soak it with 10mL Hanks eluent, and shake it on a micro-oscillator for 2 minutes to release the microorganisms in the micropores into the solution to obtain a sample concentrate; this operation requires Complete within 3 minutes;

[0031] 4) Detection of Staphylococcus aureus in samples

[0032] Mix 1mL sample concentrate with 9mL phosphate...

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Abstract

The invention provides a method for improving the detection limit of Staphylococcus aureus in easily foamable liquid food. According to the method, the sampling amount is increased, an effective defoaming agent is used for solving the problem of foaming in a sample pretreatment enrichment process, a vacuum filter membrane is adopted for sucking and filtering microorganism in a enrichment sample toa microporous filter membrane, thereby increasing the detection limit and sensitivity of the Staphylococcus aureus in the food. After the liquid food sample is treated by the method, the detection sensitivity, detection limit and detection accuracy of the Staphylococcus aureus in the liquid food are greatly improved, and the false negative result is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for improving the detection limit of Staphylococcus aureus in liquid foods that are prone to foaming. Background technique [0002] Staphylococcus aureus is a common food-borne pathogen that exists widely in nature. Staphylococcus aureus is a Gram-positive coccus that produces fat-soluble yellow pigment in the genus Staphylococcus, with a diameter of about 0.8-1.0 μm. Under microscopic conditions, it is in the shape of scattered or aggregated grapes, without spores and flagella, and most of them have no capsule. [0003] Staphylococcus aureus can easily contaminate food. According to the report of the Centers for Disease Control and Prevention in the United States, food poisoning caused by Staphylococcus aureus occupies the second place, second only to Escherichia coli, accounting for 33% of the total bacterial food poisoning. In recent years, food poisoni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/06C12R1/445
Inventor 朱海华王永王慧张立攀平洋周莉王晓瑞孙茜茜谭静李鹏冲罗蓓蓓李栋
Owner HENAN BUSINESS SCI RES INST
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