Method for rapidly forming amine group on surface of material, medical material with surface loaded with high-density amine group and application of medical material

Abstract

The invention discloses a method for rapidly forming an amine group on the surface of a material, a medical material with a surface loaded with a high-density amine group and an application of the medical material, and relates to the technical field of medical materials. The method for rapidly forming the amine group on the surface of the material comprises the steps that the to-be-loaded materialis subjected to standing in an alkaline solution to obtain an alkali-activated material; the alkali-activated material reacts with a PAMAM-NH2 solution for 5min-24h. The medical material with the surface loaded with the high-density amine group comprises the to-be-loaded material, the surface of the to-be-loaded material is loaded with PAMAM-NH2, and the medical material can be prepared through the method. The preparation method is simple and easy to operate, the reaction time is short, the density of the amine groups on the surface of the material can be effectively controlled, and the requirements of different amine group densities on the surface of the material can be met.

Description

Technical field

[0001] The present invention relates to the technical field of medical materials, and particularly relates to a method for rapidly forming amine groups on the surface of materials, medical materials with high-density amine groups on the surface, and applications thereof. Background technique

[0002] Regulating the biological function of implant materials is an important content in the research of implant materials. The introduction of functional molecules on the surface of the material is an effective means. The research is carried out to transform the inert surface into an active surface with the immobilization of biological functional molecules. basis. Ti-based biomaterials are widely used in the field of biomedicine. At this stage, functional molecules are bridged mainly through silane molecules and dopamine.

[0003] The existing method of introducing amine groups on the surface of materials mainly adopts a method of constructing a rich amine group surface, wh...

Examples

Embodiment 1

[0041] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0042] After polishing and cleaning the titanium sheet, soak it in 1mol / L potassium hydroxide solution, keep it at 70℃ for 10h, then use RO water ultrasonic cleaning twice, keep it at 70℃ for 10h, and then put the material in Dry at 50℃ for later use.

[0043] The alkali-activated material and PAMAM-NH at a concentration of 0.01 mg / mL 2 The solution (molecular weight is about 6900) was reacted for 5 minutes at a temperature of 3°C, and then washed with RO for 3 times, and the ear ball was blown dry for use.

Embodiment 2

[0045] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0046] After polishing and cleaning the titanium sheet, soak it in a 5mol / L sodium hydroxide solution, keep it at 90℃ for 14h, then use RO water ultrasonic cleaning 4 times, keep it at 90℃ for 14h, and then put the material in Dry at 50℃ for later use.

[0047] The alkali-activated material and PAMAM-NH at a concentration of 1 mg / mL 2 The solution (molecular weight is about 14300) was reacted at 10°C for 24h, then washed with RO 3 times, and the ear ball was blown dry for use.

Embodiment 3

[0049] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0050] After polishing and cleaning the titanium sheet, soak it in a 3mol / L sodium hydroxide solution, keep it at 80℃ for 12h, then use RO water ultrasonic cleaning 4 times, keep it at 80℃ for 12h, then put the material in Dry at 50℃ for later use.

[0051] The alkali-activated material and PAMAM-NH at a concentration of 0.1 mg / mL 2 The solution (molecular weight is about 6909) was reacted for 30 minutes at a temperature of 5°C, and then washed with RO for 3 times, and the ear ball was blown dry for use.

Technical field

[0001] The present invention relates to the technical field of medical materials, and particularly relates to a method for rapidly forming amine groups on the surface of materials, medical materials with high-density amine groups on the surface, and applications thereof. Background technique

[0002] Regulating the biological function of implant materials is an important content in the research of implant materials. The introduction of functional molecules on the surface of the material is an effective means. The research is carried out to transform the inert surface into an active surface with the immobilization of biological functional molecules. basis. Ti-based biomaterials are widely used in the field of biomedicine. At this stage, functional molecules are bridged mainly through silane molecules and dopamine.

[0003] The existing method of introducing amine groups on the surface of materials mainly adopts a method of constructing a rich amine group surface, wh...

Examples

Embodiment 1

[0041] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0042] After polishing and cleaning the titanium sheet, soak it in 1mol / L potassium hydroxide solution, keep it at 70℃ for 10h, then use RO water ultrasonic cleaning twice, keep it at 70℃ for 10h, and then put the material in Dry at 50℃ for later use.

[0043] The alkali-activated material and PAMAM-NH at a concentration of 0.01 mg / mL 2 The solution (molecular weight is about 6900) was reacted for 5 minutes at a temperature of 3°C, and then washed with RO for 3 times, and the ear ball was blown dry for use.

Embodiment 2

[0045] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0046] After polishing and cleaning the titanium sheet, soak it in a 5mol / L sodium hydroxide solution, keep it at 90℃ for 14h, then use RO water ultrasonic cleaning 4 times, keep it at 90℃ for 14h, and then put the material in Dry at 50℃ for later use.

[0047] The alkali-activated material and PAMAM-NH at a concentration of 1 mg / mL 2 The solution (molecular weight is about 14300) was reacted at 10°C for 24h, then washed with RO 3 times, and the ear ball was blown dry for use.

Embodiment 3

[0049] This embodiment provides a method for rapidly forming amine groups on the surface of a material, which includes the following steps:

[0050] After polishing and cleaning the titanium sheet, soak it in a 3mol / L sodium hydroxide solution, keep it at 80℃ for 12h, then use RO water ultrasonic cleaning 4 times, keep it at 80℃ for 12h, then put the material in Dry at 50℃ for later use.

[0051] The alkali-activated material and PAMAM-NH at a concentration of 0.1 mg / mL 2 The solution (molecular weight is about 6909) was reacted for 30 minutes at a temperature of 5°C, and then washed with RO for 3 times, and the ear ball was blown dry for use.

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