A diagnostic kit for prostate cancer and its preparation method and application

Abstract

The kit of the invention is used for diagnosing NPM1 antibody, the Renilla luciferase gene and the NPM1 antigen gene are recombined into a fusion vector, and the fusion vector is introduced into the mammalian cell system by using the cell transformation technology, and the luciferase-NPM1 antigen fusion protein is carried out. Expression; lyse the gene recombinant cells to obtain a luciferase-NPM1 antigen fusion protein, coat the NPM1 antigen in a 96 microtiter plate, and add the serum to be tested. The antibody in the serum will bind to the antigen on the plate and attach to the plate; the luciferase-NPM1 antigen fusion protein is added, and the luciferase-NPM1 antigen fusion protein is combined with the NPM1 antibody in the patient's serum through immunoprecipitation. And adsorbed on the plate, and finally added the luciferase substrate to detect the fluorescence intensity. According to the fluorescence intensity, the content of NPM1 antibody can be reflected, which can provide reference for the diagnosis of prostate cancer. The method is fast, simple, and highly sensitive.

Description

technical field [0001] The invention relates to a kit, in particular to a prostate cancer diagnostic kit which improves the diagnostic accuracy of prostate cancer, is fast, simple and high-sensitivity, and a preparation method and application thereof. Background technique [0002] Prostate cancer is a malignant tumor that occurs in the prostate tissue of men. It has become the second most common tumor in men worldwide and ranks sixth in cancer-related mortality. [0003] At present, the clinical diagnosis methods of prostate cancer mainly include prostate digital rectal examination (DRE), serum prostate-specific antigen (PSA) detection, rectal ultrasound detection, biopsy pathological examination and so on. [0004] Digital rectal examination is a classic technique for the diagnosis of prostate cancer. It mainly touches the prostate with the doctor's index finger to judge the size and shape of the prostate nodules, so as to determine whether there is prostate cancer. Biopsy...

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a prostate cancer diagnostic kit that improves the diagnostic accuracy of prostate cancer, is fast, simple and highly sensitive in order to solve the defects that the existing detection of prostate cancer is easy to cause misdiagnosis and missed diagnosis.