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Simple and rapid propagation method of spotted wing drosophila pupae parasitic wasp trichopria drosophilae perkins

A technology for Drosophila melanogaster and parasitic wasps, which is applied in the field of simple and rapid indoor reproduction of the parasitic wasp A. triceratops at the pupal stage of Drosophila melanogaster, can solve the pollution of Drosophila melanogaster, cannot meet the needs of mass reproduction, The process is cumbersome and complicated, and the effect is economical and easy to operate.

Inactive Publication Date: 2019-05-21
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0004] The Chinese patent with the application number CN201610213523.3 discloses a method for indoor breeding of the horny hornet, which is roughly the same as the general breeding method of the parasitic wasp in step (3) of this method, and is only suitable for keeping the parasitic samara in the laboratory Stable population of fly parasitoids; because the process of breeding Drosophila melanogaster is cumbersome and complicated, it is easy to be polluted by Drosophila melanogaster, and the number of artificially reproduced offspring is small, so the use of Drosophila melanogaster as a host cannot meet the needs of a large number of parasitoids
The Chinese patent application number CN201710749534.8 discloses a production method for the artificial breeding of pupal parasitoids that strengthens the biological control effect of fruit flies. , life expectancy and other aspects have been enhanced, but in the process of operation, manpower is needed to pick fruit fly pupae, which naturally cannot meet the needs of mass reproduction

Method used

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  • Simple and rapid propagation method of spotted wing drosophila pupae parasitic wasp trichopria drosophilae perkins
  • Simple and rapid propagation method of spotted wing drosophila pupae parasitic wasp trichopria drosophilae perkins
  • Simple and rapid propagation method of spotted wing drosophila pupae parasitic wasp trichopria drosophilae perkins

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Experimental program
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Embodiment 1

[0033] Acquisition of the species of the horned horned horned wasp:

[0034] The parasitic wasp utilized in the method—Herpes pilaris is from trapping in the field. Design of trapping device: This device is refitted from a 500ml mineral water bottle, and the body of the bottle is drilled with a small hole with a diameter of 0.5cm. For the convenience of hanging the trapping bottle on the fruit tree, the cap is drilled and tied with an iron wire. Drosophila feed containing Drosophila spotted wing eggs, larvae and pupae in each developmental stage. During the period when the berry fruit flies harm the orchard, hang the trapping device under the bayberry tree (about 1m above the ground on the shady side), observe the trapping situation every day, and bring the trapped adult parasitoid bees and the host in the device back to the insect culture room breed.

[0035] After trapping, the hosts in different periods in the device were transferred to the insect culture room with temper...

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Abstract

The invention relates to the field of biological control over pests, and provides a simple and rapid propagation method of spotted wing drosophila pupae parasitic wasp trichopria drosophilae perkins.The method mainly comprises the steps that a drosophila breeding pot and a parasitic wasp breeding pot are prepared; spotted wing drosophila and drosophila melanogaster are propagated; trichopria drosophilae perkins is generally propagated; parasitism of drosophila melanogaster pupae and parasitic wasps is prepared; parasitic wasps obtained after large-scale rearing are applied. According to the method, time is saved, labor is saved, and the device is simple, a large number of trichopria drosophilae perkins can be propagated in a short time, so that the requirements of large population experiments on the parasitic wasps are met conveniently, or application to drosophila control in the field is achieved conveniently.

Description

technical field [0001] The invention belongs to the field of biological control of pests, and in particular relates to a simple and rapid indoor breeding method for the parasitic wasp Ceratia chinensis in the pupal stage of Drosophila spotted wing. Background technique [0002] Spotted Drosophila[ Drosophila suzukii (Matsumura, 1931)] also known as Cherry Drosophila, Drosophila Suzuki, the English name is Spotted wing drosophila (SWD), belongs to Diptera (Diptera) ring split suborder (Cyclorrhapha) Drosophilidae (Drosophilidae) Drosophila genus (Drosophila) fruit fly subgenus (Sophophora) Drosophila melanogaster species group. Unlike most fruit flies that feed on rotting fruit, spotted wing Drosophila is different because the female has a hard, serrated ovipositor that can lay eggs in ripe or soon-to-ripe soft-skinned fruits. At present, it is known that the spotted-winged Drosophila can harm more than 60 kinds of fruits in 18 families, including blueberries, raspberries, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G13/00
Inventor 仪传冬林嘉刘旭祥宋学森季清娥陈家骅
Owner FUJIAN AGRI & FORESTRY UNIV
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