A simple and rapid propagation method of Drosophila spotted wing pupae parasitic hornet horn beetle

Abstract

The patent of the present invention relates to the field of pest biological control, and provides a simple and rapid breeding method for the parasitic wasp trichomes of the spotted wing Drosophila pupae. The main contents of the method are: the preparation of the Drosophila insect tank and the parasitoid bee tank; the reproduction of the spotted wing Drosophila and the Drosophila melanogaster; parasitism; application of parasitic wasps after mass rearing. The method is time-saving, labor-saving, simple and easy to install, and can multiply the scutellaria trichomes in a large amount in a short period of time, so as to meet the requirements of a large population experiment of this parasitic wasp in the laboratory or apply it to the control of fruit flies in the field.

Description

technical field [0001] The invention belongs to the field of biological control of pests, and in particular relates to a simple and rapid indoor breeding method for the parasitic wasp Ceratia chinensis in the pupal stage of Drosophila spotted wing. Background technique [0002] Spotted Drosophila[ Drosophila suzukii (Matsumura, 1931)] also known as Cherry Drosophila, Drosophila Suzuki, the English name is Spotted wing drosophila (SWD), belongs to Diptera (Diptera) ring split suborder (Cyclorrhapha) Drosophilidae (Drosophilidae) Drosophila genus (Drosophila) fruit fly subgenus (Sophophora) Drosophila melanogaster species group. Unlike most fruit flies that feed on rotting fruit, spotted wing Drosophila is different because the female has a hard, serrated ovipositor that can lay eggs in ripe or soon-to-ripe soft-skinned fruits. At present, it is known that the spotted-winged Drosophila can harm more than 60 kinds of fruits in 18 families, including blueberries, raspberries, ...

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Problems solved by technology

[0004] The Chinese patent with the application number CN201610213523.3 discloses a method for indoor breeding of the horny hornet, which is roughly the same as the general breeding method of the parasitic wasp in step (3) of this method, and is only suitable for keeping the parasitic samara in the laboratory Stable population of fly parasitoids; because the process of breeding Drosophila melanogaster is cumbersome and complicated, it is easy to be polluted by Drosophila melanogaster, and the number of artificially reproduced offspring is small, so the use of Drosophila melanogaster as a host cannot meet the needs of a large number of parasitoids

The Chinese patent application number CN201710749534.8 discloses a production method for the artificial breeding of pupal parasitoids that strengthens the biological control effect of fruit flies. , life expectancy and other aspects have been enhanced, but in the process of operation, manpower is needed to pick fruit fly pupae, which naturally cannot meet the needs of mass reproduction

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