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Metarhizium anisopliae IPPMHBC-009 complex microbial agent and application thereof

A technology of Metarhizium anisopliae and compound bacterial agent, which is applied in the directions of application, insecticide, biocide, etc., can solve the problems of unstable compound effect of strains and pesticides, reduced locust control effect, failure to improve, etc., and achieves alleviation Creates resistance, resolves lethal slowness, reduces contamination effects

Inactive Publication Date: 2019-05-21
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The compounding of bacterial strains and pesticides is an explorable way to improve the effect of locust control, but the effect of compounding of bacterial strains and pesticides is unstable at present, and improper compounding fails to improve or even reduce the effect of locust control

Method used

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  • Metarhizium anisopliae IPPMHBC-009 complex microbial agent and application thereof
  • Metarhizium anisopliae IPPMHBC-009 complex microbial agent and application thereof
  • Metarhizium anisopliae IPPMHBC-009 complex microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Bioassay of the pathogenicity of Metarhizium anisopliae IPPMHBC-009 of Scaraba spp.

[0024] Metarhizium anisopliae IPPMHBC-009 is isolated from soil, and its preservation number is CGMCC No.16000.

[0025] Toxicity of 9 strains of Metarhizium anisopliae to locusts was determined by gastric poisoning method.

[0026] First weigh 0.2g of Metarhizium anisopliae spore powder and add it to 2.0g of wheat bran, then add 0.1ml of edible oil, and stir well. Then weigh 0.2g of the prepared Metarhizium anisopliae wheat bran bait and put it into a petri dish, put it into a glass-covered insect rearing basket together with the petri dish, number them, and then put 20 of the same size into the insect rearing basket. 3rd instar hairy-legged locust gnat. No Metarhizium anisopliae spore powder was added to the wheat bran bait of the blank control group. The present invention selects 9 strains of Metarhizium anisopliae spore powder and a control CK, and each kind of spore p...

Embodiment 2

[0034] Example 2. Bioassay of the pathogenicity of Metarhizium anisopliae IPPMHBC-009 to the Asian locust

[0035] Determination of 9 bacterial strains is to the disease-curing power of Asian carcus, and assay method is the same as the method for embodiment 1.

[0036] The bioassay results of the pathogenicity of 9 strains of Metarhizium anisopliae to P. figure 2 ,Table 2.

[0037] Table 2 Comparison of the pathogenicity of 9 strains of Metarhizium anisopliae to P.

[0038]

[0039] From the analysis in Table 2, it can be seen that the tested strains are pathogenic to locust gnats, the half-lethal time is between 3.55d and 10.67d, and the rate of dead insects is between 38.33% and 93.33%. Among them, the half-lethal time of Metarhizium anisopliae strain IPPMHBC-009 was 3.55 days, and the dead insect rate was 93.33%. Compared with strain 200614, Metarhizium anisopliae strain IPPMHBC-009 has significant differences, indicating that it has higher pathogenicity to P.

Embodiment 3

[0040] Example 3 Determination of the pathogenicity of Metarhizium anisopliae IPPMHBC-009 and chemical pesticides to the Asian locust

[0041] There were 3 treatments in the experiment, each treatment contained 3 groups of 3-instar locust nymphs, and each group had 3 repetitions. The first treatment was treated with low mass concentration of 95.5% chlorantraniliprole (0.004, 0.02, 0.1ppm), the second treatment was treated with Metarhizium anisopliae IPPMHBC-009 (0.0064%, 0.032%, 0.16%), the third A mixture of low concentrations of 95.5% chlorantraniliprole and Metarhizium anisopliae IPPMHBC-009 (0.004ppm+0.0064%, 0.02ppm+0.032%, 0.1ppm+0.16%) was used for each treatment.

[0042] Treatment method: in the first treatment group, 95.5% chlorantraniliprole was diluted with acetone to obtain 0.004, 0.02, 0.1ppm; in the second treatment group, the spore powder of Metarhizium anisopliae IPPMHBC-009 was diluted with wheat bran to obtain 0.0064%, 0.032 %, 0.16% three concentrations; t...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a metarhizium anisopliae IPPMHBC-009 complex microbial agent and application thereof. The preservation number of the metarhizium anisopliae (Metarhizium Anisopliae) IPPMHBC-009 is CGMCC No.16000, and the metarhizium anisopliae IPPMGBC-009 has good median lethal time and bombys batryticatus rate for oedaleus decorus asiaticus and dasyhippus barbipes. The metarhizium anisopliae IPPMHBC-009 complex microbial agent and the application thereof have the advantage that through the combined application of the metarhizium anisopliae IPPMHBC-009 and chlorantraniliprole, the fast-acting property of killing insects and insect killing rate can be significantly improved.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a compound bacterial agent of Metarhizium anisopliae IPPMHBC-009 and its application. Background technique [0002] Locusts are disastrous insects of Orthoptera Acrididae. Locust plagues occur frequently in my country, causing great damage to my country's agriculture. At present, the main means of controlling grassland locust damage in my country is to use chemical control methods, which have the advantages of fast, efficient, and flexible use. However, as people pay more and more attention to the sustainable development of the environmental economy, biological locust control methods have been developed. and apply it to actual work. Biological locust control methods, especially Metarhizium anisopliae, Beauveria bassiana, etc., have certain specificity, are harmless to humans and animals, and also have the advantages of no environmental pollution, no residue, and n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/56A01P7/04
Inventor 王广君李贝贝农向群涂雄兵张泽华
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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