A kind of biological material and its preparation method and its application as bone defect repair material

A technology of biomaterials and bioceramic materials, applied in the field of biomaterials and their preparation, and bone defect repair materials, can solve the problems of limited printing height, yellowing of materials, and low printing accuracy of biomaterials

Active Publication Date: 2020-09-08
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention solves the problem of light-induced cross-linking molding of the opaque system of inorganic bioceramic particles in the prior art, as well as the technical problems of low printing accuracy of biological materials, limited printing height and yellowing of materials

Method used

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  • A kind of biological material and its preparation method and its application as bone defect repair material
  • A kind of biological material and its preparation method and its application as bone defect repair material
  • A kind of biological material and its preparation method and its application as bone defect repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: C-CS 0.4g+Gel-MA 2.2g+HAP 5g+photocrosslinking+EDC / NHS crosslinking

[0033] The preferred embodiment of the present invention provides an extrusion type 3D printing biological material for bone defect repair. The biological 3D printing slurry formula includes an organic polymer part, an inorganic bioceramic particle part and a photoinitiating additive part.

[0034] The organic polymer part is composed of Gel-MA and C-CS. Take the C-CS 0.4g and dissolve it in 11mLH under magnetic stirring 2 After adding 2.2 g of the Gel-MA in O, it was completely dissolved under stirring in a water bath at 50°C.

[0035] The photoinitiator additive partly consists of photoinitiator 819 and photoinitiator 184. Take the 0.15g photoinitiator 819 and 0.25g photoinitiator 184, and dissolve them in 0.4 mL of acetone to completely dissolve, and the solution becomes slightly yellow. The prepared photo-initiating additive is partially added to the organic polymer solution, and magneticall...

Embodiment 2

[0039] Example 2: C-CS 0.8g+Gel-MA 3.2g+photocrosslinking

[0040] In the biological 3D printing slurry component for bone repair of the present invention, the organic polymer part can be printed independently without relying on inorganic particles.

[0041] Take 0.8 g of the C-CS and dissolve it in 11 mL of water with magnetic stirring, then add 3.2 g of the Gel-MA, and dissolve completely in a water bath at 50°C. The photoinitiator additive partly consists of photoinitiator 819. Take 0.15 g of the photoinitiator 819 and dissolve it in 0.4 mL of acetone until it is completely dissolved.

[0042] The prepared photo-initiating additive is partially added to the organic polymer solution, and magnetically stirred until it is completely miscible to form a uniform solution.

[0043] Transfer the slurry to the extrusion-type bioprinter barrel, match the needle with 0.16mm aperture, set the air pressure to about 0.15MPa, the line speed 6mm / s, the line spacing set 0.3mm, and the 330-365nm ul...

Embodiment 3

[0044] Example 3: C-CS 0.4g+Gel-MA 2.2g+HAP 3g+glutaraldehyde crosslinking

[0045] Take 0.4g of the C-CS and dissolve it in 11mL H 2 After adding 2.2 g of the Gel-MA in O, it was completely dissolved in a water bath at 50°C.

[0046] The part of the inorganic bioceramic particles (hydroxyapatite) was sieved twice with a standard sieve with an aperture of 0.15 mm, and 3 g of the sieved particles were taken. The inorganic bioceramic particles were added to the above-mentioned homogeneous solution in five times. After each addition, Put it into a planetary centrifugal mixer and mix for about 15 minutes. After the last 3g is added, mix for 1 hour to obtain a uniform and stable slurry.

[0047] Transfer the slurry to the extruded bio-printer barrel, match the needle with 0.41mm aperture, set the air pressure to about 0.4MPa, the line speed 8mm / s, the line spacing setting 1.1mm, the platform temperature 4℃, the barrel temperature 30℃ To print. After printing, put the stent into 1.25% gl...

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Abstract

The invention discloses a biological material, a preparation method thereof and application of the biological material as a bone defect repairing material and belongs to the technical field of medicalbiological materials. The preparation method comprises the steps of adding a photoinitiator solution into an aqueous solution containing methacrylic anhydride gelatin, wherein the photoinitiator solution is a photoinitiator 819 solution or a mixed solution of the photoinitiator 819 and a photoinitiator 184; carrying out extrusion type 3D biological printing on printing slurry, and carrying out ultraviolet irradiation on the slurry when the slurry is extruded; preferably, the aqueous solution containing methacrylic anhydride gelatin also contains carboxymethyl chitosan; preferably, chemical crosslinking is carried out after printing is finished; preferably, an inorganic bioceramic material is added to the printing slurry. The method has the advantages of simple components, stable printingprocess, high precision, high crosslinking degree, high crosslinking speed and good scaffold biocompatibility, and the biological material can also be used as a carrier of medicines or other functional components, and has very wide application prospect in the field of bone defect repair.

Description

Technical field [0001] The invention belongs to the technical field of medical biomaterials, and more specifically, relates to a biomaterial, a preparation method thereof, and an application as a bone defect repair material. Background technique [0002] Bone damage caused by accidents and aging usually requires implantation of bone repair materials during surgical treatment. However, autologous bone transplantation can cause secondary trauma, and allogeneic bone transplantation may cause immune response. The research on synthetic bone defect repair materials appears to be very important and has a wide range of clinical application requirements. Bone defect repair materials often use inorganic bioceramic particles (such as hydroxyapatite, bioactive glass, etc.) combined with polymers (gelatin, collagen, polylactic acid, etc.) to make porous scaffolds. These porous scaffolds can promote bone The ability of tissues to grow into and achieve osseointegration can also be degraded aft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/22A61L27/20A61L27/10A61L27/12A61L27/50A61L27/54C08J3/24C08J3/28B33Y70/10B33Y80/00C08L89/00C08L5/08
Inventor 马军石玉飞
Owner HUAZHONG UNIV OF SCI & TECH
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