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Preparation method of notoginseng polysaccharide

The technology of Panax notoginseng polysaccharide and Panax notoginseng is applied in the field of preparation of Panax notoginseng polysaccharide, which can solve the problems of low polysaccharide content, difficulty in separation and extraction, lack of in-depth understanding of polysaccharide and the like

Inactive Publication Date: 2019-05-31
威海紫光生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low content of polysaccharides in natural products, the difficulty of separation and extraction, and the complex structure, there is still a lack of in-depth understanding of the molecular mechanism of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Soak the crushed Panax notoginseng in absolute ethanol to remove oil and pigment, air-dry the dregs, boil in water for 60 rain, centrifuge, add distilled water to the precipitate and boil for 60 min, repeat 2-3 times, discard the precipitate, and combine the supernatant (brown yellow), concentrated under reduced pressure, added 3 volumes of 95% ethanol to the supernatant, allowed to stand for 24 h, and centrifuged to collect the precipitate. The precipitate was washed twice with absolute ethanol and acetone, drained with a suction filter funnel, dried with P2O5, and neutral protease combined with Sevag method to deproteinize crude polysaccharide.

[0011] The crude polysaccharide was dissolved in PBS, centrifuged to remove insoluble matter, and the supernatant was chromatographically separated through a DEAE-Sepharose Fast Flow anion chromatographic column, and eluted with a gradient of 0-0.8 mol / L NaCL-PBS solution (pH7.6) to The absorbance value at 490 nm (A490) was m...

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Abstract

The invention relates to a preparation method of notoginseng polysaccharide. The preparation method includes following steps: smashing, soaking, removing grease and pigment, boiling in water for centrifuging, alcohol extracting, draining, and chromatographically separating. Notoginseng content in extract obtained by the preparation method is far higher than that of conventional methods, and content of other ingredients and impurities is lower than that of conventional extraction.

Description

technical field [0001] The invention relates to a preparation method of an extract, in particular to a preparation method of notoginseng polysaccharide. Background technique [0002] For more than ten years, scholars at home and abroad have extracted many polysaccharides with immune regulation and anti-tumor activities from medicinal plants, and some of them have entered the clinical stage and are used in the recovery of immunity after chemotherapy and radiotherapy. The research on carbohydrates is now focused on the recognition and binding of biological information receptors in the immune system, which is of special significance to pharmacology, that is, the immunomodulatory effect. However, due to the low content of polysaccharides in natural products, the difficulty of separation and extraction, and the complex structure, there is still a lack of in-depth understanding of the molecular mechanism of polysaccharides. [0003] Polysaccharides are polymers in which aldose an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王阳光
Owner 威海紫光生物科技开发有限公司
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