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A kind of identification method of Indian moringa and Pteris moringa in Moringa leaves

A technology of moringa and moringa, which is applied in the identification of moringa and moringa in leaves of Moringa oleifera, in the field of compound identification, can solve the problem that the curative effect of medicines cannot be guaranteed or cannot be effectively guaranteed Product quality and other issues

Active Publication Date: 2020-12-01
济南维瑞医药科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality of the product cannot be effectively guaranteed during production or use, so the efficacy of the drug cannot be guaranteed

Method used

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  • A kind of identification method of Indian moringa and Pteris moringa in Moringa leaves
  • A kind of identification method of Indian moringa and Pteris moringa in Moringa leaves
  • A kind of identification method of Indian moringa and Pteris moringa in Moringa leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 The water extract of Moringa oleifera leaves was purified with LSA5B macroporous adsorption resin, and a large amount of pigments, sugars and water-soluble proteins were removed with water, and then eluted with chloroform, and the chloroform eluate was concentrated as a test solution. Take Indian moringa reference substance and Pteris moringa reference substance respectively and dissolve them in chloroform as the reference substance solution. Spot the test solution and reference solution on the same silica gel HF254 thin-layer chromatographic glass plate with a capillary tube. Water (3.5:2: 0.03:0.5) is used as the developing agent to expand twice, the first expansion to 1 / 2 of the front edge of the thin-layer plate, and the second expansion to the front edge of the thin-layer plate, take it out and dry it at 105°C and spray it to improve Bismuth iodide-potassium alkaloid exclusive chromogenic agent, inspected under sunlight.

Embodiment 2

[0016] Example 2 The water extract of Moringa oleifera leaves was purified with LSA5B macroporous adsorption resin, and a large amount of pigments, sugars and water-soluble proteins were removed with water first, and then eluted with chloroform, and the chloroform eluate was concentrated as the test solution. Dissolve Indian moringan reference substance and Pteris moringa reference substance in chloroform respectively as reference substance solution. Spot the test solution and the reference solution on the same silica gel HF254 thin-layer chromatographic glass plate with a capillary tube, pre-saturate with ammonia vapor at room temperature for 25 minutes, and then add chloroform-ethyl acetate-glacial acetic acid- Water (3.5:2: 0.03:0.5) is used as the developing agent to expand twice, the first expansion to 1 / 3 of the front edge of the thin-layer plate, the second expansion to 4 / 5 of the front edge of the thin-layer plate, take it out and dry it at 105°C After spraying with an...

Embodiment 3

[0017] Example 3 The water extract of Moringa oleifera leaves was purified with LSA5B macroporous adsorption resin. Firstly, a large amount of pigments, sugars and water-soluble proteins were removed with water, and then eluted with chloroform. The chloroform eluate was concentrated and used as the test solution. Dissolve Indian moringan reference substance and Pteris moringa reference substance in chloroform respectively as reference substance solution. Spot the test solution and reference solution on the same silica gel HF254 thin-layer chromatographic glass plate with a capillary tube, pre-saturate with ammonia vapor at room temperature for 15 minutes, and then add chloroform-ethyl acetate-glacial acetic acid- Water (3.5:2: 0.03:0.5) is used as the developing agent to expand twice, the first expansion to 2 / 3 of the front edge of the thin-layer plate, the second expansion to the front edge of the thin-layer plate, take it out and dry it at 105°C and spray it to improve Bismu...

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Abstract

The invention relates to a method for identifying pterygospermin and spirochin in horseradish tree leaves. A horseradish tree leaf aqueous extract is purified by LSA5B macroporous adsorption resin; and then identification is carried out by using a thin layer chromatography method at a temperature of 0 to 10 DEG C. According to the provided method, with the horseradish tree leave preparation, the drug quality and curative effect are ensured effectively in production and application.

Description

technical field [0001] The invention relates to a method for identifying compounds, in particular to a method for identifying moringa and moringa in moringa leaves, and belongs to the technical field of medicines. Background technique [0002] Moringa ( Moringa oleiferam Lam.) is native to India and Africa, and is a plant of the genus Moringa in the Moringaceae family. At present, it is widely planted in tropical and subtropical regions of Asia and Africa. Because of its rapid growth, rich nutrition and high medicinal value, it is known as the "miracle tree" and "treasure box of medicine". [0003] Moringa leaves are rich in nutrients and medicinal ingredients. Studies have shown that Moringa leaf powder has medicinal effects such as antibacterial and anti-inflammatory. Its active ingredients are mainly alkaloids, and the content of alkaloids in leaves is significantly higher than that in stems. The highest total alkaloid is 4.62mg / g. Studies have found that the alkaloid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 邵爱霞孟祥娟解洪福
Owner 济南维瑞医药科技开发有限公司
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