Living plant preservation method
A preservation method and plant technology, applied in the field of agriculture, can solve problems such as quality decline, dehydration, wilting, cell rupture, etc., to achieve the effect of ensuring freshness, clear main band of DNA, and improving detection efficiency
Inactive Publication Date: 2019-06-04
YUAN LONGPING HIGH TECH AGRI CO LTD
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AI Technical Summary
Problems solved by technology
[0004] At present, most of the corn materials for extracting DNA are fresh or frozen materials. For long-distance sampling in the field or off-site sampling of winter planting materials in Hainan, most of them are stored in a low-temperature preservation method; fresh detached leaves may lose water and wither during transportation, or due to The cell ruptures, and its contents flow out under the action of DNase, which degrades the DNA and reduces its quality
Method used
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Experimental program
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Embodiment 1
[0059] Example 1 Fast, efficient, high-quality sampling and preservation of corn leaves
[0060] 1. Experimental consumables and instruments
[0061] Sample rack with lid ( figure 1 ), sample tubes, corn leaf sampler, sample rack storage box (foam box, Figure 4 ), biological ice packs ( Figure 5 ), sponge board ( Figure 8 ), silica desiccant pack ( Figure 9 ).
[0062] For a schematic diagram of inserting a 4ml blank sample tube into a sample rack, see figure 2 .
[0063] Described sponge plate is black, and its material is PU (polyurethane) sponge, and thickness is 5mm.
[0064] 2. Sampling and preservation of corn leaves
[0065] (1) Sampling: According to the sampling order of the samples, use the sampler ( image 3 ) to sample fresh corn leaves ( Figure 7 ) in the blank sample tube on the sample rack. See leaf size Image 6 .
[0066] (2) Sample rack processing: when a sample rack is finished sampling, place the sponge plate above the sample tube, and co...
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The invention provides a living plant preservation method, which comprises the following steps: placing collected living plants or part of living plants in a sample tube, placing the sample tube intoa sample holder with a lid, placing a sponge plate above the sample tube, covering the lid of the sample holder, placing the lid-covered sample holder into a ziplock bag with a desiccant and sealing the bag and storing. By the method, freshness and quality of plants (especially leaves) for long-distance transport at normal temperature can be effectively improved. The method can be used for laboratory molecular marker and other molecular biology experimental sampling, and provides genomic DNA samples of high-quality plants (especially corn leaves) for various types of detection.
Description
technical field [0001] The invention relates to the field of agricultural technology, in particular to a living plant preservation method. Background technique [0002] Corn is an important grain, economy, and feed crop. It is also the main raw material for the pharmaceutical, oil, and alcohol industries. It plays a very important role in the world's economic life. However, with the continuous development of plant science to the molecular level, high-quality DNA samples are often required during the breeding and improvement of maize varieties. Genomic DNA samples extracted from young and fresh corn leaves have uniform concentration, high yield, and good quality; however, as the leaves continue to grow, primary metabolites such as phenols, polysaccharides, lipids, etc. Accumulation of DNA, the relative reduction of contained DNA, coupled with long-distance transportation, makes DNA extraction more difficult, lower in purity, and lower in quality. Therefore, to exclude the i...
Claims
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IPC IPC(8): A01N3/00
Inventor 邹继军谈存梅伊凡·舒斯特尔玛丽安娜·罗沙·多托劳拉·克里斯蒂娜·卡尔加罗卢东林王建海李晓鹏阮祥经
Owner YUAN LONGPING HIGH TECH AGRI CO LTD