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A kit and detection method for detecting pb2+ concentration

A kit and concentration technology, applied in color/spectral characteristic measurement, material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as inability to evaluate, and achieve the effect of simple method, good recognition and response ability, and high sensitivity

Active Publication Date: 2020-04-28
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method has a strong detection ability for the concentration of lead ions, but neither the existing instrumental analysis methods nor biosensors can evaluate other pollution-related information (such as pH value) of lead ions in the sample well. There are certain limitations in the sample analysis of

Method used

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  • A kit and detection method for detecting pb2+ concentration
  • A kit and detection method for detecting pb2+ concentration
  • A kit and detection method for detecting pb2+ concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Pb in the neutral condition of embodiment 1 2+ Drawing of detection standard curve

[0051] Take 9 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7, 8 and 9, and do the following:

[0052] Add 940 μL of reagent II to No. 1 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L of reagent I, so that the solution volume is 1mL, to obtain solution 1;

[0053] Add 937.5 μL of reagent II to No. 2 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII and 2.5 μL 100×10 -6 mol / L reagent IV, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;

[0054] Add 935 μL of reagent II to No. 3 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII and 5 μL 100×10 -6 mol / L reagent IV, mix well and incubate at 25°C for 20...

Embodiment 2

[0064] Pb in the acidic condition of embodiment 2 2+ Drawing of detection standard curve

[0065] Take 11 EP tubes, numbered 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11, and do the following:

[0066] Add 940 μL of reagent III to No. 1 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L of reagent I, so that the solution volume is 1mL, to obtain solution 1;

[0067] Add 939.5 μL of reagent III to No. 2 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII and 0.5 μL 100×10-6 mol / L reagent IV, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;

[0068] Add 939 μL of reagent III to No. 3 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII and 1 μL 100×10 -6 mol / L reagent IV, mix well and incubate at 25...

Embodiment 3

[0081] Carry out Pb under the condition of embodiment 1 2+ Concentration measurement, verification detection method of the present invention detects Pb 2+ ability to concentrate.

[0082] Take 3 EP tubes, labeled 1, 2 and 3, and do the following:

[0083] Add 932.5 μL of reagent II to No. 1 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L reagent VII and 7.5 μL 100×10 -6 mol / L reagent IV, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 The reagent I of mol / L, make the solution volume be 1mL, obtain solution 1;

[0084] Add 930 μL of reagent II to No. 2 EP tube, and then add 20 μL with a concentration of 100×10 -6 mol / L of reagent VII and 10 μL 100×10 -6 mol / L reagent IV, mix well and incubate at 25°C for 20 min, then add 40 μL to a concentration of 100×10 -6 mol / L reagent 1, make the solution volume 1mL, obtain solution 2;

[0085] Add 922.5 μL of reagent II to No. 3 EP tube, and then add 20 μL with a concentr...

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Abstract

The invention provides a kit used for detecting the concentration of Pb2+ and a detection method. The kit comprises the following components: cyanine dyes, a Pb2+ standard solution, DNA double strands, an acidic buffer solution and a neutral buffer solution. The detection method comprises the steps of respectively drawing Pb2+ detection standard curves under neutral and acidic conditions; mixing asample to be detected, the DNA double strands and the cyanine dyes, incubating the mixed solution at a temperature of 20-40 DEG C for 15-25min, then measuring the absorbance at 448nm or the fluorescence intensity at 616nm of the mixed solution, judging the acidity of the sample to be detected according to the detected absorbance or fluorescence intensity, and then calculating the concentration ofPb2+ by combining the Pb2+ detection standard curve under the neutral or acidic condition. The kit is simple in system, and the detection method is high in sensitivity, low in detection limit and high in accuracy. Meanwhile, the acidity of the sample to be detected can also be reflected, and thus a simple method is provided for judging the migration rate of lead ions.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, in particular to a method for detecting Pb 2+ Concentration kits and detection methods. Background technique [0002] Metal lead has excellent physical and chemical properties. Thousands of years ago, people discovered that it is easy to mine, soft and easy to process. At present, lead is widely used in industries such as alloys, building materials and batteries. The toxicity of lead has been concerned since the late 19th century, mainly manifested as neurotoxicity, cardiovascular toxicity and reproductive toxicity, and the harm to children is especially serious. Its toxicological mechanism is to destroy the normal configuration of the enzyme, so that the enzyme loses its catalytic function; it interferes with the metabolism of metal ions in the human body, thereby affecting the normal physiological functions of cells. As an important pollutant in environmental monitoring, mon...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/64
Inventor 杨千帆杨舒杨春容李济丞杨冬林苗家榕姚烨
Owner SICHUAN UNIV
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