Cage-structured laser scanning confocal microscopic imaging system and method

Abstract

The invention discloses a cage-structured laser scanning confocal microscopic imaging system and method. The system mainly comprises a three-dimensional precision sample stage, a microscope objectivelens, a fluorescence microscope tube, a cage-structured optical machine component, a laser, a single mode fiber, an optical fiber beam splitter, a dichroic mirror, a filter, an imaging CCD, a single-photon detector, a pulse counter, a calibrator and the like. The main body of the microscopy system is in a stable cage-structured, is assembled from the easy-to-install cage-structured optical machinecomponent, and can optimize excitation laser and fluorescence at different wavelengths. The single mode fiber is used for spatial filtering. The single-photon detector and the pulse counter are usedto detect a weak fluorescence signal at the single-photon level. The calibrator is used to carry out single-photon property analysis on a fluorescence light source. The system provided by the invention has the advantages of high integration, small occupation volume, easy installation and debugging, simple operation and maintenance and the like, and can be used for high-precision imaging of weak fluorescence signals and high-efficiency collection and signal analysis of single-photon sources.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a laser scanning confocal microscopic imaging system and method with a cage structure. Background technique [0002] Laser confocal microscope is a common scientific research equipment for studying fluorescence imaging and weak fluorescence signal collection, detection and signal analysis, and it is also widely used in biomedical and industrial detection fields. For the detection and analysis of some weak fluorescent signals, collection efficiency and detection efficiency are crucial. [0003] The current commercial laser confocal microscope generally has a complex structure, takes up a large space, and has too many optical elements, which reduces the collection efficiency and detection efficiency of fluorescent signals. A laser scanning confocal microscopy imaging system based on a cage structure provided by the present invention aims to minimize the use of optical el...

AI Technical Summary

Problems solved by technology

[0003] The current commercial laser confocal microscope generally has a complex structure, occupies a large space, and has too many optical elements, which reduces the collection efficiency and detection efficiency of fluorescent signals.

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