Cage-structured laser scanning confocal microscopic imaging system and method

A technology of confocal microscopy and cage structure, applied in microscopes, optics, optical components, etc., can solve the problems of reducing fluorescence signal collection efficiency and detection efficiency, occupying a large space, and many optical components, etc., to improve signal-to-noise ratio, easy assembly and disassembly, and simplified structural design

Active Publication Date: 2019-06-04
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

[0003] The current commercial laser confocal microscope generally has a complex structure, occupies a large space, and has too many optical elements, which reduces the collection efficiency and detection efficiency of fluorescent signals.

Method used

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  • Cage-structured laser scanning confocal microscopic imaging system and method
  • Cage-structured laser scanning confocal microscopic imaging system and method
  • Cage-structured laser scanning confocal microscopic imaging system and method

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Embodiment Construction

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0037] According to attached Figure 1-2 Shown, the present invention is further described:

[0038] figure 1 It is a schematic diagram of the device structure provided by an embodiment of the present invention. Such as figure 1 as shown,

[0039] The upper side of the microscope objective lens is a fluorescent microscope barrel with a cage structure, which is composed of a laser incident module in the horizontal direction and a fluorescence co...

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Abstract

The invention discloses a cage-structured laser scanning confocal microscopic imaging system and method. The system mainly comprises a three-dimensional precision sample stage, a microscope objectivelens, a fluorescence microscope tube, a cage-structured optical machine component, a laser, a single mode fiber, an optical fiber beam splitter, a dichroic mirror, a filter, an imaging CCD, a single-photon detector, a pulse counter, a calibrator and the like. The main body of the microscopy system is in a stable cage-structured, is assembled from the easy-to-install cage-structured optical machinecomponent, and can optimize excitation laser and fluorescence at different wavelengths. The single mode fiber is used for spatial filtering. The single-photon detector and the pulse counter are usedto detect a weak fluorescence signal at the single-photon level. The calibrator is used to carry out single-photon property analysis on a fluorescence light source. The system provided by the invention has the advantages of high integration, small occupation volume, easy installation and debugging, simple operation and maintenance and the like, and can be used for high-precision imaging of weak fluorescence signals and high-efficiency collection and signal analysis of single-photon sources.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a laser scanning confocal microscopic imaging system and method with a cage structure. Background technique [0002] Laser confocal microscope is a common scientific research equipment for studying fluorescence imaging and weak fluorescence signal collection, detection and signal analysis, and it is also widely used in biomedical and industrial detection fields. For the detection and analysis of some weak fluorescent signals, collection efficiency and detection efficiency are crucial. [0003] The current commercial laser confocal microscope generally has a complex structure, takes up a large space, and has too many optical elements, which reduces the collection efficiency and detection efficiency of fluorescent signals. A laser scanning confocal microscopy imaging system based on a cage structure provided by the present invention aims to minimize the use of optical el...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00G01N21/64
Inventor 李强王俊峰闫飞飞程则迪许金时李传锋
Owner UNIV OF SCI & TECH OF CHINA
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