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Zwitter ionic micro gels, their assemblies and related formulations, and methods for their use

A zwitterion and microgel technology, applied in the field of cell culture, can solve different biocompatibility, difficulty, expensive and other problems

Pending Publication Date: 2019-06-04
UNIV OF WASHINGTON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polysaccharide gels have many inherent limitations, which have poor long-term physiological stability, different biocompatibility, and are difficult and expensive to purify from natural sources or synthesize into medical-grade materials

Method used

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  • Zwitter ionic micro gels, their assemblies and related formulations, and methods for their use
  • Zwitter ionic micro gels, their assemblies and related formulations, and methods for their use
  • Zwitter ionic micro gels, their assemblies and related formulations, and methods for their use

Examples

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Embodiment 1

[0212] Preparation, Characterization and Use of Representative Zwitterionic Microgels

[0213] In this example, methods for preparing, characterizing, and using representative zwitterionic microgels of the invention are described.

[0214] Microgel Production . To prepare zwitterionic microgel constructs, a photopolymerization method was used to prepare bulk zwitterionic hydrogels. Carboxybetaine acrylamide (CBAA) monomer (2.5M), CBAA-X crosslinker (0.01-1%mol / mol) and photoinitiator 2hydroxy-1-[4-(2-hydroxyethoxy )phenyl]2-methyl-1-propanone (I2959) was dissolved in water, mixed well, and degassed under vacuum. The concentrated solution was then cast into 1 mm thick glass molds and polymerized in a Spectroline XL-1500UV oven. The resulting hydrogel is equilibrated in water for several days to remove any unreacted reagents and allow it to swell. Libraries of these hydrophilic gels were generated using CBAA-1 or CBAA-2 monomers and several CBAA-X crosslinker concentrati...

Embodiment 2

[0220] Preservation of platelets with representative zwitterionic microgels

[0221] In this example, platelet preservation using representative zwitterionic microgels of the invention is described.

[0222] Platelets are blood cells that play a key role in blood clotting and have many other functions. Platelet transfusions are mandatory in trauma and blood disorders. Unfortunately, when removed from the donor's bloodstream and stored, platelets become activated and quickly become useless. Current prior art solutions require room temperature storage under constant gentle agitation to prevent aggregation and even allow oxygen diffusion. Cold storage (4°C) will cause platelets to lose their coagulation ability even faster, but the maximum storage time at room temperature is only between 5-7 days. Although platelet supplement solutions and gas permeable bags have increased this maximum storage time, non-specific aggregation, bacterial contamination and platelet interaction w...

Embodiment 3

[0229] Bioreactor

[0230] In this example, biomanufacturing or industrial scale cell culture or expansion using representative zwitterionic microgels of the invention is described.

[0231] Currently, there is limited success in expanding stem cell populations and other cells relevant to immunotherapy such as T cells in conventional bioreactors while maintaining their pluripotency and / or therapeutic activity. Most materials present in reactors, including optimized biomaterials and modified surfaces, provide nonspecific interactions with cells that provoke phenotypic changes, promote cellular senescence, or require destructive encapsulation reactions and recovery procedures. Shear damage in stirred tank reactors also limits growth. Pure zwitterionic hydrogels maintain stem cell pluripotency for unprecedented lengths of time and support their expansion at small scales while protecting them from shear damage. To support cell expansion over long periods of time in continuous ...

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Abstract

Zwitter ionic micro gels, zwitter ionic micro gel assemblies, their formulations and methods for their use are disclosed.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Application No. 62 / 365,788 filed July 22, 2016, which is expressly incorporated herein by reference in its entirety. [0003] Statement of Government Licensing Rights [0004] This invention was made with government support under awards DMR1307375 and CMMI1301435 from the National Science Foundation and N00014-15-1-2277 from the Office of Naval Research. The government owns certain rights in inventions. Background technique [0005] Hydrogels are hydrated elastic polymer networks that share many properties with natural tissues. Many of their growing biomedical applications, including cosmetic procedures, topical therapy delivery, and regenerative cell scaffolds, require injectability or malleability to avoid invasive procedures or to fill unique three-dimensional (3D) volumes. Among the natural and synthetic polymers used to construct hydrogels, polyzwitterions have received s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/30A61K38/02A61K31/711
CPCA01N1/0215A01N25/04A61Q19/00A61K47/30A61K8/042A61K2800/10A61L2202/00A61K31/704A61K35/19A61L27/34A61L27/52A61L27/54A61L2300/62A61L2300/64A61L2400/06A01N1/0231
Inventor 江绍毅A·辛克莱尔M·E·奥可莉白涛P·简恩
Owner UNIV OF WASHINGTON
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