Dual cycle tissue engineering blood vessel in vitro culture system for simulating pulsation blood flow
A technology of tissue engineering and in vitro culture, which is applied in the direction of tissue cell/virus culture device, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc. Require high-level problems to achieve the effects of simplified structure, convenient operation and reduced cost
Active Publication Date: 2019-06-07
GUIZHOU UNIV
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Problems solved by technology
[0005] However, most of the current pulsating culture equipment is imported equipment, the equipment structure is complex, the operation requirements are high, and the cost is expensive.
Method used
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[0022] The present invention will be further described below in conjunction with the examples, but not as a basis for limiting the present invention.
[0023] Embodiments of the invention
[0024] A dual-cycle tissue engineering blood vessel culture system in vitro that simulates pulsating blood flow, as shown in the attached Figure 1-2 As shown, it includes a square culture chamber 1, the symmetrical sides of the culture chamber 1 are respectively connected with a first pulse circulation tube 2 and a second pulse circulation tube 3, and the first pulse circulation tube 2 or the second pulse circulation tube 3 is provided with There is a pulsating peristaltic pump 4, and the first medium circulation pipe 5 and the second medium circulation pipe 6 are respectively connected to the symmetrical two sides, and the first medium circulation pipe 5 or the second medium circulation pipe 6 is provided with a circulating peristaltic pump. Pump 7 ; the other ends of the first pulsating...
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The invention discloses a dual cycle tissue engineering blood vessel in vitro culture system for simulating pulsation blood flow. The dual cycle tissue engineering blood vessel in vitro culture systemcomprises a square culture cavity, wherein a first pulsating circulating pipe and a second pulsating circulating pipe are respectively connected to two symmetrical sides of the culture cavity, and apulsation peristaltic pump is arranged on the first pulsating circulating pipe or the second pulsating circulating pipe. In addition, a first culture medium circulating pipe and a second culture medium circulating pipe are respectively connected to two symmetrical sides, and a circulation peristaltic pump is arranged on the first culture medium circulating pipe or the second culture medium circulating pipe; and the other ends of the first pulsating circulating pipe, the second pulsating circulating pipe, the first culture medium circulating pipe and the second culture medium circulating pipe are all connected to the inner part of a culture medium circulating bottle. The system has the characteristics of being simple in structure, simple to operate, low in cost, and good in culture effects.
Description
technical field [0001] The invention relates to an in vitro culture system of tissue engineering blood vessels, in particular to a dual-cycle tissue engineering blood vessel in vitro culture system simulating pulsating blood flow. Background technique [0002] In recent years, due to the westernization of lifestyle and the acceleration of the aging process of the social population, the incidence of cardiovascular disease has been increasing year by year, which poses a serious threat to human health and social and economic stability. According to the World Health Organization statistics, cardiovascular disease has become the main cause of human death. Coronary and peripheral vascular disease is the largest cause of death and requires surgical procedures, including small-diameter bypass grafts with native veins or arteries. However, many patients lack sufficient autologous blood vessels for bypass grafting. Vascular grafts made of artificial synthetic materials have a low pa...
Claims
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Login to View More IPC IPC(8): C12M3/06C12M1/04
Inventor 李龙李兴茂黄琳石雁
Owner GUIZHOU UNIV