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Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone

A high-efficiency technology for degrading bacteria and single cells, which is applied in the field of biotechnology to degrade pesticide residues and achieves remarkable effects, good lasting effect, and environmental friendliness.

Pending Publication Date: 2019-06-07
GUIZHOU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

So far, no research work has been carried out on the biodegradation of sclerotin

Method used

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  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone
  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone
  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone

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Embodiment 1

[0018] 1. Screening of immobilized carrier:

[0019] Preliminary experiments showed that in 1%, 2%, 3%, 4%, 5% sodium alginate (SA) immobilized and suspended free cells, 4% SA showed a better effect. Furthermore, the experiment measured the degradation effect of free cells, bamboo charcoal (BC) + free cells, 4% SA + free cells, 4% SA + bamboo charcoal (BC) + free cells on 75 mg / L of Sclerotium. The results are shown in figure 1 .

[0020] 2. Preparation of immobilized bacteria:

[0021] 1) Preparation of bacterial suspension: under aseptic conditions, add 2 mL of sterile phosphate buffer to the culture dish for 48 hours of culture and shake in parallel, and gently scrape off the colonies on the surface of the medium with an inoculating loop to form a crude bacterial suspension Transfer it to a liquid fermentation medium (maltose 7g / L, yeast extract 6g / L, NaCl 3g / L), and culture for 48-56h at a pH of 7.5-8, 30-35°C and 150-200rpm. The removed fermentation broth was immediately centr...

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Abstract

The invention discloses a preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone. Brevundimonas naejangsanensis CCTCC M 2017739 is used as a strain, and 4% sodium alginate and bamboo charcoal are used as immobilization materials, in order to efficiently immobilize the Brevundimonas naejangsanensis strain to prepare immobilized bacteria. The immobilized bacteria can be applied to efficient and thorough degradation of Dimetachlone, vinclozolin and iprodione in soil and water, and has the advantages of good persistence,environmental friendliness, remarkable effect, low cost and easy promotion.

Description

Technical field [0001] The invention relates to the field of biotech degradation of pesticide residues, in particular to a preparation method and application of immobilized bacteria of the genus Sclerotium that efficiently degrade Sclerotium. Background technique [0002] Pesticides play an important role in the comprehensive management of agricultural diseases and insect pests, but pesticide residues and their metabolites have become serious pollutants, posing a threat to human health and ecosystems. Dimetachlone, also known as Wenkuli, molecular formula C 10 H 7 Cl 2 NO 2 , Its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl) succinimide. It is widely used in the prevention and treatment of fungal diseases such as sclerotium, sheath blight, brown spot and other crops on rapeseed, Chinese cabbage, laver, lettuce, leeks, cucumber, tomato, pepper, potato, tobacco and other crops in China. Although Sclerotium has a promising prospect of bacterici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10A01N25/32C09K17/14C02F3/34C12R1/01C02F101/38
Inventor 吴小毛张承李姣红龙友华李荣玉黄文源王秋萍代园凤
Owner GUIZHOU UNIV
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