Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone

A high-efficiency technology for degrading bacteria and single cells, which is applied in the field of biotechnology to degrade pesticide residues and achieves remarkable effects, good lasting effect, and environmental friendliness.

Pending Publication Date: 2019-06-07
GUIZHOU UNIV
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no research work has been carried out on the biodegradation of sclerotin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone
  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone
  • Preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Screening of immobilized carrier:

[0019] Preliminary experiments showed that in 1%, 2%, 3%, 4%, 5% sodium alginate (SA) immobilized and suspended free cells, 4% SA showed a better effect. Furthermore, the experiment measured the degradation effect of free cells, bamboo charcoal (BC) + free cells, 4% SA + free cells, 4% SA + bamboo charcoal (BC) + free cells on 75 mg / L of Sclerotium. The results are shown in figure 1 .

[0020] 2. Preparation of immobilized bacteria:

[0021] 1) Preparation of bacterial suspension: under aseptic conditions, add 2 mL of sterile phosphate buffer to the culture dish for 48 hours of culture and shake in parallel, and gently scrape off the colonies on the surface of the medium with an inoculating loop to form a crude bacterial suspension Transfer it to a liquid fermentation medium (maltose 7g / L, yeast extract 6g / L, NaCl 3g / L), and culture for 48-56h at a pH of 7.5-8, 30-35°C and 150-200rpm. The removed fermentation broth was immediately centr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method and application of immobilized Brevundimonas naejangsanensis bacteria highly efficient in degrading Dimetachlone. Brevundimonas naejangsanensis CCTCC M 2017739 is used as a strain, and 4% sodium alginate and bamboo charcoal are used as immobilization materials, in order to efficiently immobilize the Brevundimonas naejangsanensis strain to prepare immobilized bacteria. The immobilized bacteria can be applied to efficient and thorough degradation of Dimetachlone, vinclozolin and iprodione in soil and water, and has the advantages of good persistence,environmental friendliness, remarkable effect, low cost and easy promotion.

Description

Technical field [0001] The invention relates to the field of biotech degradation of pesticide residues, in particular to a preparation method and application of immobilized bacteria of the genus Sclerotium that efficiently degrade Sclerotium. Background technique [0002] Pesticides play an important role in the comprehensive management of agricultural diseases and insect pests, but pesticide residues and their metabolites have become serious pollutants, posing a threat to human health and ecosystems. Dimetachlone, also known as Wenkuli, molecular formula C 10 H 7 Cl 2 NO 2 , Its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl) succinimide. It is widely used in the prevention and treatment of fungal diseases such as sclerotium, sheath blight, brown spot and other crops on rapeseed, Chinese cabbage, laver, lettuce, leeks, cucumber, tomato, pepper, potato, tobacco and other crops in China. Although Sclerotium has a promising prospect of bacterici...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10A01N25/32C09K17/14C02F3/34C12R1/01C02F101/38
Inventor 吴小毛张承李姣红龙友华李荣玉黄文源王秋萍代园凤
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap