A blood cell lysate and method for extracting nucleic acid in blood using the lysate

A blood cell and lysate technology, applied in biochemical equipment and methods, microbial measurement/inspection, DNA preparation, etc., can solve problems such as low efficiency, affecting follow-up tests, and easy contamination, achieving high yield and saving detection time , the effect of not easy to pollute
CN109852611BActive Publication Date: 2021-07-23BEIJING NAGENE DIAGNOSTIC REAGENT CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING NAGENE DIAGNOSTIC REAGENT CO LTD
Publication Date
2021-07-23

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Abstract

The invention discloses a blood cell lysate and a method for extracting nucleic acid in blood by using the lysate. The blood cell lyse contains heparin magnetic beads with a mass fraction of 3% to 10%, and Triton X-100 with a mass fraction of 0.5 to 1.5%. , urea with a final concentration of 0.5-1.0M, mass fraction of 0.02-0.08% sodium lauroyl sarcosinate, and calcium chloride and / or magnesium chloride with a final concentration of 0.1-0.3M. The compound detergent in the lysate of the present invention can effectively lyse cells, and the heparin magnetic beads can effectively adsorb proteins, and under the action of calcium (magnesium) ions, PCR interfering substances such as proteins can be solidified rapidly and nucleic acids can be separated out by warm treatment. Nucleic acids of high purity are obtained by centrifugation. The lysate of the present invention is stable in nature and temperature, and is not affected by seasons, temperatures, salt ion concentrations, etc., and the method is simple and fast in operation, is not easy to be polluted, has a high yield of nucleic acid extraction, and is suitable for clinical and scientific research.
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Description

technical field

[0001] The invention relates to the field of molecular biology detection, in particular to a blood cell lysate and a method for extracting nucleic acid in blood using the lyse. Background technique

[0002] Nucleic acid is the carrier of genetic information, the most important biological information molecule, and the main object of molecular biology research. Therefore, nucleic acid extraction is the most important and basic operation in molecular biology experimental techniques. Nucleic acid extraction refers to the process of separating nucleic acid from the carrier by physical and chemical methods. At present, most medical institutions and scientific research fields in China need to extract nucleic acid from a large number of blood samples. Therefore, in order to ensure that high-concentration nucleic acids are extracted, new technologies are constantly being developed.

[0003] There are many methods for nucleic acid extraction, including the traditiona...

Claims

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