HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint

A fingerprint, ethyl acetate technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve good separation effect, rich chromatographic peak information, and improve the standard of medicinal materials.

Active Publication Date: 2019-06-07
GUANGXI UNIV OF CHINESE MEDICINE
View PDF16 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to further improve the standards of longan leaf medicinal materials, promote the standardization of traditional Chinese medicine, ensure and improve the quality of longan leaf medicinal materials, and provide accurate and reliable evidence for the safety and e

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint
  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint
  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiment, but do not limit protection scope and application scope of the present invention:

[0030] The invention provides a method for establishing the HPLC fingerprint of the ethyl acetate part of longan leaves, comprising the following steps:

[0031] S1. Preparation of the test solution: take 2 g of longan leaf coarse powder, accurately weigh it, put it in a stoppered conical flask, first add 20ml of 95% ethanol for ultrasonication for 1h, then add 20ml of 50% ethanol for ultrasonication for 30min, filter, and evaporate the filtrate to dryness , add water to suspend in a separatory funnel, add an equal amount of petroleum ether and repeatedly extract until the extracted petroleum ether layer is colorless, take the lower layer solution, add an equal amount of ethyl acetate and repeatedly extract until the extracted ethyl acetate layer is colorless color, combined the ethyl acetate l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses an HPLC fingerprint for the ethyl acetate position of a longan leaf and an establishing method of the HPLC fingerprint. The method comprises the steps that a solution of the tested object is prepared; a contrast solution including ethyl gallate, astragalin and quercetin is prepared; and HPLC is used for determination to obtain the fingerprint. The method has the advantagesof being rapid, accurate, high in precision, repeatability and stability, abundant in chromatographic peak information, and good in separating effect. The established HPLC fingerprint for the ethyl acetate position of the longan leaf can effectively represent the quality of the longan leaf, especially the quality of the ethyl acetate position of the longan leaf, and furhter the medical standard ofthe longan leaf is improved, the longan leaf quality evaluation system is improved, standardization of traditional Chinese medicines is facilitated, theoretical and practical basis is provided for comprehensive and effective control for the longan leaf quality, and accurate reliable basis is provided for the safety and effectiveness of clinical medicine.

Description

technical field [0001] The quality control method of the traditional Chinese medicinal material of the invention specifically relates to a method for constructing a high-performance liquid chromatography fingerprint of the ethyl acetate part of longan leaves and a standard fingerprint. Background technique [0002] Longan leaf is a traditional Chinese medicinal material with Guangxi national characteristics, which was first recorded in "Southern Yunnan Bencao Tushuo". It is the leaves or shoots of longan belonging to Sapindaceae longan. Guangxi, Guangdong, Fujian and Taiwan are the main producing areas, and Hainan, Yunnan, Sichuan and Guizhou are also cultivated. Its nature and flavor are sweet, light, and flat, and it has the functions of clearing heat, dampness and detoxification. It is mainly used to treat cold, fever, malaria, boils, eczema, etc. Longan leaves mainly contain tannins, phenolic acids, flavonoids and volatile oils. At present, there are not many domestic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
CPCY02A50/30
Inventor 梁洁黄春燕周昱杉孙正伊徐晖麦嘉妮
Owner GUANGXI UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap