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Adjustable calibration-fluorescence detection cell on refraction compensation column

A fluorescence detection and detection cell technology, applied in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve the problems of inability to take into account the sealing of the cell body, the disappearance of the refractive compensation effect, and the leakage of glycerin, so as to ensure long-term The effect of stability, better alignment, and high detection signal-to-noise ratio

Active Publication Date: 2019-06-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this structure cannot take into account the tightness of the cell body while being adjustable. After dripping glycerin, it can only maintain a limited refraction compensation time, such as 15 minutes. After the time exceeds, glycerin will slowly leak along the gap, making the refraction compensation effect gradually disappear.

Method used

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  • Adjustable calibration-fluorescence detection cell on refraction compensation column
  • Adjustable calibration-fluorescence detection cell on refraction compensation column
  • Adjustable calibration-fluorescence detection cell on refraction compensation column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] An adjustable calibration-refractive compensation laser-induced fluorescence detector, the excitation light source is a 450nm small semiconductor laser diode. The excitation fiber 13 and the excitation fiber ferrule structure 10 are coaxially placed in the ground groove of the pool base 1; the core diameter of the excitation fiber 13 is 50 μm, the numerical aperture is 0.22, and the distance between the excitation fiber 13 and the transparent light window 8 of the capillary is 50 μm; The inner diameter is 50 μm, the outer diameter is 365 μm, and the capillary 7 vertically passes through the center line of the cavity of the pool base 1; the collecting optical fiber 9 has a core diameter of 0.4 mm, a numerical aperture of 0.22, and an included angle of 45° with the capillary 7, and the center lines of the two are coplanar, One end of the collection fiber 9 is in physical contact with the transparent light window 8; the sockets of the capillary 7 and the collection fiber 9 ...

Embodiment 2

[0045] The fluorescence detection cell structure as described in Example 1, the excitation light source was replaced by a 470nm blue LED, and the detection sample was fluorescein isothiocyanate (FITC).

[0046] Experimental results: The calculated detection limit is 1.5×10 -10 M.

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Abstract

The invention provides an adjustable calibration-fluorescence detection cell on a refraction compensation column. Specifically, adjustable parts in the detection cell are mechanically bridged with a three-dimensional displacement platform, and excitation optical fiber-light window-collection optical fiber system is subjected to micron-scale three-dimensional calibration by matching observation holes in the periphery of the cell; meanwhile, the detection cell is tightly sealed by using sealing rings, a clamping sleeves and a blocking head, so that the long-term stability of a refraction compensation system is ensured after glycerin is dripped, and finally high detection sensitivity is obtained. The detection cell can independently replace device parameters, such as the inner and outer diameters of capillary tubes, excitation optical fibers and collection optical fibers, different application requirements can be adapt to.

Description

technical field [0001] The patent of the present invention relates to a fluorescence detection device, more specifically, to an adjustable calibration-fluorescence detection cell on a refraction compensation column. Background technique [0002] Fluorescence detection has extremely high sensitivity and plays an important role in ultratrace analysis. The detection cell is an important part of the detection system, and its performance determines the detection sensitivity. By designing and optimizing the structure of the detection cell, the stray light background of the excitation light of the system can be reduced, and the collection efficiency of fluorescence can be improved, so that a highly sensitive detection system can be obtained. [0003] At present, the on-column detection cell is the most widely used cell structure, which has the advantages of small extra-column effects and strong versatility. However, there is an obvious optical interface (quartz capillary n=1.467 / ...

Claims

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Application Information

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IPC IPC(8): G01N21/03G01N21/64
Inventor 耿旭辉关亚风宁海静高岩
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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