The invention provides a preparation method and application of a copper selenide nanosheet array for a sodium ion battery with adjustable interlayer spacing. The copper selenide nanosheet array is made of high-purity copper foil, a selenium source, sodium hydroxide, A strong reducing agent and an intercalation agent are used as raw materials, water is used as a solvent, and a reaction is performed at 20-80° C. for 0.5-4 h to obtain a copper selenide nanosheet array for sodium-ion batteries with adjustable layer spacing. The prominent feature of the present invention is that the distance between copper selenide layers can be regulated at a lower temperature, and the preparation method of the material is simple, the energy consumption is low, and it is easy to industrialized large-scale production; Controllable appearance and high specific surface area. When the material is used as an anode material for a sodium ion battery, it exhibits the advantages of high specific capacity, good rate performance and superior cycle performance. Therefore, the material is expected to be widely used in sodium ion batteries and other alkali metal ion batteries, thermoelectric materials and other fields.