Preparation method and application of boric acid functionalized graphene material with targeted photo-thermal sterilization performance

A carboxylated graphene and photothermal technology, which is applied to medical preparations with no active ingredients, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the secondary damage of surrounding normal tissues, photothermal The agent has no targeting ability to bacteria and cannot be killed by heating, so as to achieve the effect of less toxic and side effects, realize targeted killing, and treat skin infection

Inactive Publication Date: 2019-06-14
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the key problem of current photothermal therapy is that the photothermal agent has no targeting ability to bacteria, and cannot be combined with pathogenic bacteria to directly kill them by heating, and it is difficult to avoid secondary damage to surrounding normal tissues.

Method used

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  • Preparation method and application of boric acid functionalized graphene material with targeted photo-thermal sterilization performance
  • Preparation method and application of boric acid functionalized graphene material with targeted photo-thermal sterilization performance
  • Preparation method and application of boric acid functionalized graphene material with targeted photo-thermal sterilization performance

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Experimental program
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Embodiment 1

[0036] Embodiment one: a kind of preparation method of the boric acid functionalized graphene material with targeted photothermal sterilization performance, it comprises the following steps:

[0037] Step 1: uniformly mix 2mg carboxylated graphene, 0.6mg EDC, 0.6mg NHS and 10mL deionized water, and stir at room temperature for 4 hours to obtain a mixed solution;

[0038] Step 2: 2 mg of 3-aminopyridine was added to the mixture, and stirred at room temperature for 24 hours to obtain suspension A;

[0039] Step 3: Centrifuge the suspension A at a speed of 11,000 rpm for 90 minutes, then repeatedly wash the product 3 times with deionized water to remove impurities;

[0040] Step 4: Resuspend the product obtained in Step 3 with 10 mL of DMF to obtain a suspension B, add 2 mg of 3-bromomethylphenylboronic acid to the suspension B, and stir for 12 hours in an oil bath at a temperature of 50°C , to obtain suspension C;

[0041] Step 5: Centrifuge the suspension C at a speed of 13,0...

Embodiment 2

[0042]Embodiment two: a kind of preparation method of the boric acid functionalized graphene material with targeted photothermal sterilization performance, it comprises the following steps:

[0043] Step 1: uniformly mix 2mg carboxylated graphene, 0.6mg EDC, 0.6mg NHS and 10mL deionized water, and stir at room temperature for 8 hours to obtain a mixed solution;

[0044] Step 2: 2 mg of 3-aminopyridine was added to the mixture, and stirred at room temperature for 24 hours to obtain suspension A;

[0045] Step 3: Centrifuge the suspension A at a speed of 11,000 rpm for 90 minutes, then repeatedly wash the product 3 times with deionized water to remove impurities;

[0046] Step 4: Resuspend the product obtained in Step 3 with 10 mL of DMF to obtain a suspension B, add 2 mg of 3-bromomethylphenylboronic acid to the suspension B, and stir for 6 hours in an oil bath at a temperature of 50°C , to obtain suspension C;

[0047] Step 5: Centrifuge the suspension C at a speed of 13,000...

Embodiment 3

[0048] Embodiment three: a kind of preparation method of the boric acid functionalized graphene material with targeted photothermal sterilization performance, it comprises the following steps:

[0049] Step 1: uniformly mix 2mg carboxylated graphene, 0.6mg EDC, 0.6mg NHS and 10mL deionized water, and stir at room temperature for 4 hours to obtain a mixed solution;

[0050] Step 2: 2 mg of 3-aminopyridine was added to the mixture, and stirred at room temperature for 24 hours to obtain suspension A;

[0051] Step 3: Centrifuge the suspension A at a speed of 11,000 rpm for 90 minutes, then repeatedly wash the product 3 times with deionized water to remove impurities;

[0052] Step 4: Resuspend the product obtained in Step 3 with 10 mL of DMF to obtain a suspension B, add 2 mg of 3-bromomethylphenylboronic acid to the suspension B, and stir for 12 hours in an oil bath at a temperature of 90°C , to obtain suspension C;

[0053] Step 5: Centrifuge the suspension C at a speed of 13...

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Abstract

The invention discloses a preparation method and an application of a boric acid functionalized graphene material with targeted photo-thermal sterilization performance, and the preparation method comprises the following steps: step 1, uniformly mixing carboxylatedgraphene, EDC, NHS and deionized water, and stirring at room temperature; step 2, adding 3-aminopyridine into the mixed liquor, and stirring at room temperature; step 3, performing centrifugal treatment, and washing with deionized water; step 4, resuspending the product obtained in step 3 with DMF, and adding 3-phenylboronic acid, andstirring under oil bath conditions; step 5, carrying out centrifugal treatment and washing by using deionized water to obtain the boric acid functionalized graphene material with the targeted photo-thermal sterilization performance. The preparation method has the advantages of high-efficiency targeting bacteria and capability of photothermal killing bacteria, good biocompatibility and simple preparation method; the preparation method can effectively treat skin wound surface infection of multiple drug-resistant Gram-negative bacilli and promote healing of skin infection wound surface.

Description

technical field [0001] The invention relates to a functionalized graphene material, in particular to a preparation method and application of a boric acid functionalized graphene material with targeted photothermal sterilization performance. Background technique [0002] Bacterial infection of skin wounds has always been a major challenge in the treatment of burns and wounds. At present, bacterial infections still mainly rely on antibiotic treatment, but clinical antibiotic treatment is often accompanied by the emergence and prevalence of multi-drug resistant strains. Due to the lack of new antibiotics, some Pan-drug-resistant strains, especially multidrug-resistant Gram-negative bacilli, even appear to be "drug-resistant". Multidrug-resistant Gram-negative bacilli infection in skin wounds has become a thorny problem in clinical anti-infection treatment. Because traditional antibiotic treatment is difficult to work, we urgently need to find some new treatment strategies to so...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/54A61P31/04A61P17/00
Inventor 钱卫王鹤罗高兴贺伟峰张小容王颖胡晓红赵保华
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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