Preparation method of polyethylenimine grafted rice bran polysaccharide iron and gene vector
The technology of polyethyleneimine and rice bran polysaccharide is applied in the field of gene carriers, which can solve the problems of restricting the progress and development of gene carriers, limited transfection efficiency, complicated preparation reaction, etc., and achieves the promotion of coagulation efficiency and stability, low reaction condition requirements, High biocompatibility effect
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[0041] For this reason, the present invention proposes a kind of preparation method of polyethyleneimine grafted rice bran polysaccharide iron, is used for preparing above-mentioned polyethyleneimine grafted rice bran polysaccharide iron, combines figure 1 The schematic flow chart of an embodiment of the preparation method of polyethyleneimine grafted rice bran polysaccharide iron shown, the preparation method of described polyethyleneimine grafted rice bran polysaccharide iron comprises the following steps:
[0042] Step S10, under an inert atmosphere, sequentially add triethylamine and N,N-carbonyl-diimidazole solution to the rice bran polysaccharide iron solution, and react in the dark to form a reaction solution.
[0043] Wherein, after triethylamine is added to the rice bran polysaccharide iron solution, the pH of the rice bran polysaccharide iron solution is 8-9; and the light-shielding reaction time is 3-5 hours.
[0044]In an alkaline environment, rice bran polysacchar...
Embodiment 1
[0066] Dissolve 1g of rice bran polysaccharide iron in 80ml of dimethyl sulfoxide to obtain rice bran polysaccharide iron solution, and set aside; take 2.5g of N,N-carbonyl-diimidazole and dissolve it in 40ml of dimethyl sulfoxide to obtain N,N-carbonyl- Diimidazole solution, for later use; Dissolve 8 g of polyethyleneimine in 100 ml of dimethyl sulfoxide to obtain polyethyleneimine solution, for later use.
[0067] Under the protection of nitrogen, add triethylamine and 4ml of N,N-carbonyl-diimidazole solution to 8ml of the above-mentioned rice bran polysaccharide iron solution in turn to form a solution to be reacted (pH of the solution to be reacted is 8.0), and stir the reaction in a dark environment 3h to form a reaction solution; within 1h, 10ml of polyethyleneimine solution was added dropwise to the above reaction solution, and at the same time, triethylamine was added dropwise to the reaction solution to control the pH to be 8.8, and the reaction was stirred for 22h und...
Embodiment 2
[0069] Take 1g rice bran polysaccharide iron and dissolve it in 90ml dimethyl sulfoxide to get rice bran polysaccharide iron solution, and set aside; take 2.2g N,N-carbonyl-diimidazole and dissolve it in 40ml dimethyl sulfoxide to get N,N-carbonyl- Diimidazole solution, set aside; Dissolve 8.5g polyethyleneimine in 100ml dimethyl sulfoxide to obtain polyethyleneimine solution, set aside.
[0070] Under the protection of nitrogen, add triethylamine and 4ml of N,N-carbonyl-diimidazole solution to 9ml of the above rice bran polysaccharide iron solution in turn to form a solution to be reacted (pH of the solution to be reacted is 9.0), and stir the reaction in a dark environment 5h to form a reaction solution; within 1h, 10ml of polyethyleneimine solution was added dropwise to the above reaction solution, and at the same time, triethylamine was added dropwise to the reaction solution to control the pH to be 8.9, and the reaction was stirred for 23h under dark conditions to obtain ...
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