Bacterial culture detection method

Abstract

The invention discloses a bacterial culture detection method. The method comprises the following steps: (1) inserting a photosensitive chip at the bottom of a culture bottle; wherein the photosensitive chip comprises a CO2 / pH sensing chip and an O2 partial pressure sensing chip, and a fluorescent glue layer wrapped on the surface of the sensing chip; wherein the fluorescence intensity of a first emitted light excited by the CO2 / pH sensing the chip increases as the pH of a culture solution decreases; the fluorescence intensity of a second emitted light excited by the O2 partial pressure sensingchip increases as the O2 content in the culture solution decreases; (2) placing the bacteria and the culture solution in the culture bottle, and then introducing the growth gas and sealing; (3) adjusting the temperature of the culture bottle as needed; (4) irradiating the photosensitive chip with the light source through the culture solution; and (5) collecting the fluorescence intensity of the first emitted light and the second emitted light at different time points; and determining the change of the content of the bacteria in the culture solution according to the change of the fluorescenceintensity. The bacterial culture detection method has the advantages of simple steps, low cost, low operation difficulty, good detection effect and high accuracy.

Description

technical field [0001] The invention relates to a bacterial culture detection method. Background technique [0002] The culture and detection of bacteria is an indispensable inspection item in daily work in the fields of medical and health, inspection and quarantine, food safety, and disease control. [0003] At present, the steps of the method for bacterial culture detection are relatively complicated, the cost is high, and the operation is difficult. Contents of the invention [0004] The purpose of the present invention is to provide a bacterial culture detection method with simple steps, low cost, low operation difficulty, good detection effect and high accuracy. [0005] In order to achieve the above object, the technical scheme adopted in the present invention is: [0006] A bacterial culture detection method, comprising the following steps: [0007] (1) Put a photosensitive chip at the bottom of the culture bottle; [0008] The photosensitive chip includes CO 2...

AI Technical Summary

Problems solved by technology

[0003] At present, the method steps for bacterial culture detection are relatively complicated, the cost is high, and the operation is difficult

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