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Preparation method of sweet bean halves with high enzyme activity capacity

A technology of enzyme activity and ability, which is applied in the field of preparation of Douban sweet petals with high enzyme activity, can solve the problems of not being able to produce enzymes of compound strains, too many strains, and bad metabolites, so as to improve enzyme production ability, More umami, better quality

Inactive Publication Date: 2019-06-18
成都市旺丰食品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

201811045903.6 discloses a compound strain fermented watercress and its preparation method. This invention improves the defect of insufficient enzyme activity of a single strain. However, after a variety of different strains are combined, due to different growth conditions of microorganisms, different strains may exist Competition under the same fermentation conditions cannot exert the highest enzyme production ability of the composite strain
And if there are too many strains, other bad metabolites may be produced, which will affect the flavor of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of Doubanzi with high enzyme activity, comprising the following steps:

[0037] (1) Raw material screening: check and accept commercially available broad beans according to the acceptance criteria, including removing stone impurities, moldy or incomplete broad beans;

[0038] (2) Pretreatment: remove the outer skin of the collected broad beans to obtain broad bean flaps, first soak them in warm drinking water at 35°C for 40 minutes, and then soak them in hot water at 60°C for 30 minutes. Fold into a bow shape, pick up and drain, and set aside;

[0039] (3) Koji making: Put 10kg of commercially available flour, 3kg of mixed strains and 200kg of soaked broad bean split into the mixing tank for 3 times, keep the stirring condition at 200r / min for 10min, after stirring evenly, spread the koji material, Control the thickness of the material to 10cm, then control the temperature to 35°C, pH value to 6.5, and time to 48h, so that the microbial strains can...

Embodiment 2

[0047] A preparation method of Doubanzi with high enzyme activity, comprising the following steps:

[0048] (1) Raw material screening: check and accept commercially available broad beans according to the acceptance criteria, including removing stone impurities, moldy or incomplete broad beans;

[0049] (2) Pretreatment: remove the outer skin of the collected broad beans to obtain broad bean flaps, soak them in warm drinking water at 37°C for 45 minutes, and then soak them in hot water at 65°C for 35 minutes. Fold into a bow shape, pick up and drain, and set aside;

[0050] (3) Koji making: Put 12kg of commercially available flour, 4kg of mixed strains and 220kg of soaked broad bean flap into the mixing tank for 3 times, keep the stirring condition at 220r / min for 12min, after stirring evenly, spread the koji material, Control the thickness of the material to 15cm, then control the temperature to 36°C, pH value to 6.7, and time to 47h, so that the microbial strains can multip...

Embodiment 3

[0058] A preparation method of Doubanzi with high enzyme activity, comprising the following steps:

[0059] (1) Raw material screening: check and accept commercially available broad beans according to the acceptance criteria, including removing stone impurities, moldy or incomplete broad beans;

[0060] (2) Pretreatment: remove the outer skin of the collected broad beans to obtain broad bean flaps, soak them in warm drinking water at 40°C for 50 minutes, and then soak them in hot water at 70°C for 40 minutes. Fold into a bow shape, pick up and drain, and set aside;

[0061] (3) Koji making: Put 15kg of commercially available flour, 5kg of mixed strains and 250kg of soaked broad bean flap into the mixing tank for 3 times, keep the stirring condition at 250r / min for 15min, after stirring evenly, spread the koji material, Control the thickness of the material to 20cm, then control the temperature to 37°C, pH value to 6.8, and time to 50h, so that the microbial strains can multip...

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PUM

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Abstract

The invention discloses a preparation method of sweet bean halves with high enzyme activity capacity. The method comprises the steps as follows: broad beans are mixed with flour and strains after being peeled, the mixture is stirred uniformly for three times, fermentation is performed under the condition of PH value of 6.5-6.8 for 48-50 h, and fermented bean halves are obtained; table salt and drinking water are added to the fermented bean halves, the mixture is mixed uniformly, fermentation is performed at 38-40 DEG C for 170-180 d, deep turning and uniform stirring are performed every 10 d,broad bean cloves can be taken out of a tank after the broad bean halves are maroon according to sensory standard, and mature sweet bean halves are obtained. Single microbial strain (aspergillus oryzae No.1) with vigorous metabolic activity is obtained by repeated microbial inoculation and screening in combination with ultraviolet radiation promoted microbial variation, fermentation under the conditions of 35-37 DEG C and PH value of 6.5-6.8 is combined, the enzyme producing capacity of sweet bean halve fermentation strains is improved fundamentally, so that sweet bean halves with higher activity of enzymes such as protease and amylase are obtained, and broad bean paste products have better umami and better quality.

Description

technical field [0001] The invention relates to the field of condiment processing, in particular to a method for preparing Doubanzi with high enzyme activity. technical background [0002] Doubanjiang uses broad beans as the main raw material, and then through the action of microorganisms, and after a period of fermentation, it forms a traditional fermented food with bright red color, strong sauce aroma, spicy and salty taste. The production process can be divided into three major steps: preparation of sweet petals; preparation of pepper embryos; mixed fermentation to form bean paste. These three stages all seriously affect the quality of the final product Doubanjiang. Among them, the preparation of sweet petals is the most important, because in this stage, the value-added of microbial strains and the generation of enzyme products required for fermentation are involved. [0003] Bean paste is made from broad beans, and the nutrients in broad beans are mainly protein and ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L11/00A23L11/50
Inventor 代长安何伦何兴玉周敏张伟黎明
Owner 成都市旺丰食品有限责任公司
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