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Method for mixed fermentation of biological feed by using pomace and distiller's grains as raw materials

A technology of mixed bacteria fermentation and biological feed, which is applied in animal feed, animal feed, application, etc., can solve the problem of less Agaricus bisporus, and achieve the effects of cost saving, nutritional value improvement, and good fermentation effect

Inactive Publication Date: 2019-03-22
江苏春之雨生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the liquid fermentation technology of Agaricus bisporus is relatively mature, but there are few studies on the mixed fermentation of Agaricus bisporus and other types of microorganisms, so this will also be the research focus of the present invention

Method used

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  • Method for mixed fermentation of biological feed by using pomace and distiller's grains as raw materials
  • Method for mixed fermentation of biological feed by using pomace and distiller's grains as raw materials
  • Method for mixed fermentation of biological feed by using pomace and distiller's grains as raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. A biological feed mixed bacteria fermentation method using pomace and distiller's grains as raw material, the method may further comprise the steps:

[0052] (1) Preparation of seed solution

[0053] Agaricus bisporus: Inoculate Agaricus bisporus strains on the PDA slant medium, culture at a constant temperature of 26°C for 5-7 days, select the slant surface with white, thick and pollution-free mycelia, and use the inoculation shovel to select the activated Agaricus bisporus slant strains Take 3-4 pieces of 0.5cm on the shovel 2 Bacteria blocks were inoculated into seed liquid medium (the formula of seed liquid medium is: bran powder 5g / L, soybean powder 5g / L, sucrose 30g / L, bean sprout juice 14ml / L, potassium dihydrogen phosphate 2.0g / L, Magnesium Sulfate 1.0g / L, Calcium Chloride 0.4g / L, Cobalt Chloride 0.3mg / L, Sodium Molybdate 0.4mg / L, pH value natural) in the Erlenmeyer flask, the liquid volume is 100mL / 250mL, vibrated on a constant temperature shaker at 26°C...

Embodiment 2

[0062] Embodiment 2 (optimization test of inoculation ratio in the preparation process of mixed bacteria fermentation broth)

[0063] The method for preparing seed liquid according to embodiment 1, the total inoculation amount of Agaricus bisporus, Trichoderma reesei, Saccharomyces cerevisiae is 10%, and the inoculation ratio is adjusted to be 2:5:3, 3:4:3, 4:3:3, 5:3:2, other conditions remain unchanged, prepare the mixed bacteria fermentation liquid, measure mycelia biomass, protease activity and cellulase activity, the results are as follows figure 1 with figure 2 shown.

[0064] For mixed-bacteria fermentation, the proportion of strains inoculated has a significant impact on the final product, and choosing an appropriate inoculation ratio can make each strain play a synergistic role in the fermentation process. from figure 1 with figure 2 It can be seen that when the strain ratio is 5:3:2, the mycelium biomass, protease activity and cellulase activity of the mixed-ba...

Embodiment 3

[0065] Example 3 (optimization test of Agaricus bisporus inoculation time in advance during the preparation of mixed bacteria fermentation broth)

[0066] The seed liquid was prepared according to the method of Example 1, the advance inoculation time of the Agaricus bisporus seed liquid was set to 0, 1d, 2d, 3d, the total culture time was kept 6d, and other conditions were constant, and the mixed bacteria fermentation liquid was prepared, and the bacteria were measured. Silk biomass, protease activity and cellulase activity, the results are as follows image 3 with Figure 4 shown.

[0067] from image 3 with Figure 4It can be seen that when the inoculation time of Agaricus bisporus seed solution was 48 hours in advance, the mycelial biomass, protease activity and cellulase activity of the mixed-bacteria fermentation broth all reached the highest values. Agaricus bisporus is a large fungus. Its mycelium grows slower than Trichoderma reesei and Saccharomyces cerevisiae, an...

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Abstract

The invention provides a method for mixed fermentation of a biological feed by using pomace and distiller's grains as raw materials, and belongs to the field of industrial by-product reuse. The methodcomprises the steps of preparing seed liquid, preparing mixed fermentation broth, stimulating the mixed fermentation broth and preparing a biological feed. According to the method, edible fungus agaricus bisporus is adopted to undergo mixed fermentation with mold and saccharomycetes for the first time, and under suitable culture conditions, the funguses are mutually beneficial and symbiotic to jointly complete the metabolic activities, thereby greatly improving the fermentation efficiency and the raw material conversion rate, remarkably improving the nutritive value of the fermented feed. According to the method, a liquid solid state fermentation mode is adopted, and the advantages of the two modes are combined to comprehensively utilize the pomace and the distiller's grains, so as to provide a direction for the large-scale production of the biological feed.

Description

technical field [0001] The invention belongs to the field of reuse of industrial by-products, and in particular relates to a biological feed mixed bacteria fermentation method using pomace and distiller's grains as raw materials. Background technique [0002] Pomace is a by-product of the fruit juice processing industry. It is highly biodegradable and will cause a rotten smell and affect water resources and ecosystems. Therefore, it is necessary to find a reasonable way of resource utilization. According to statistics, the fruit pomace produced in our country can reach more than tens of thousands of tons every year, but only a small amount of it can be used for transformation and utilization. The value and utility are low, and it is not easy to preserve and can only be discarded. Such a move will undoubtedly cause huge pollution to the natural environment, and at the same time waste valuable resources such as cellulose. Studies have shown that the use of microorganisms to f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/38
CPCA23K10/12A23K10/37A23K10/38Y02P60/87
Inventor 陈甲子孙倩
Owner 江苏春之雨生物科技发展有限公司
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